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BCH 2333 Introduction to Biochemistry Lab 3 Corrected Enzymes
The purpose of experiment 1 was to determine the optimum concentration of enzymes to be used in fixed assays that give you the most constant rate of reaction over 10 mins. The purpose of experiment 2 was to determine the kinetic parameters (KM and Vmax) for the enzyme acid phosphatase using the Hanes plot. The purpose of experiment 3 was to determine the identity of an inhibitor by comparing the kinetic parameters (KM and Vmax) to that of the controls
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