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BCH 2333 Introduction to Biochemistry Lab 5 Corrected Nucleic Acids
This lab aimed to display some of the fundamental molecular principles involved in double stranded DNA and to quantify and characterize the DNA using UV spectrophotometry at different wavelengths and by using a melting curve. The purity and the effects of low and high salt on the DNA were also investigated. A plasmid map was constructed to map the relative position of the HindIII and EcoRI restriction enzyme cut sites on a plasmid following digestion of the plasmid with these two enzymes. This map was made by analysing the migration distances of the resulting fragments by agarose gel electrophoresis
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