Notesale: Turn your study into money

BCH 2333 Amino Acids and Proteins Laboratory Introduction to Biochemistry

The first objective was to separate a glycine, aspartic acid and lysine mixture using an ion-exchange chromatography. The pH of the sixteen fractions resulting from the separation was obtained. These pH values along with the elution volume helped illustrate how through a gradient of increasing pH, the amino acid were eluted based on their isoelectric point. A Ninhydrin test followed by the heating of the amino acid samples was also performed to assess the concentration of the amino acid by spectrophotometry. The second objective was to find the molecular weight of an unknown protein using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). As the unknown protein separated by electrophoresis, the molecular weight markers traced the migration of the protein along the gel. The end result of that made it possible to model the linear relationship between the logarithm of the protein molecular weight and its migration distance. By interpolation, the molecular weight of the unknown protein was then found.