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Bio 260 (Microbiology) Final Exam 2024. All Questions & Answers.
Bio 260 (Microbiology) Final Exam 2024. This study guide provides all questions and correct answers for the Bio 260 Microbiology final exam. Designed to help students excel in their microbiology course, it covers essential topics, ensuring thorough preparation for the exam.
£16.25 Preview RemoveBCH 2333 Enzymes Laboratory Introduction to Biochemistry
This lab looked at the enzymatic action of acid phosphatase in catalyzing the hydrolysis of phosphoric acid monoesters bond. The phosphoric acid bond of interest in this case was that of a substrate known as p-nitrophenylphosphate (PNPP). Once hydrolyzed by acid phosphatase, PNPP was converted to p-nitrophenol (PNP) product and an inorganic bisulfate anion. This lab came with three experiments. In the first experiment, the purpose was to determine the desired concentration of acid phosphatase that would keep the rate of hydrolysis constant for a ten minutes period. In the second experiment, the purpose was to use a lineweaver burk plot to find two kinetic parameters, Km and Vmax. Lastly, in the third experiment, an inhibitor was added to the hydrolysis reaction and the purpose was to determine the identity of the inhibitor. This was achieved by comparing the Km and Vmax values for the inhibited reaction to that of a control sample. The number of the inhibitor was 22 and the backup data were used.
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