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Title: Proteins
Description: All about the structure of proteins from amino acids to polypeptides, how they all come together and the tests.
Description: All about the structure of proteins from amino acids to polypeptides, how they all come together and the tests.
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Proteins
Proteins are very large molecules
The monomers that make up proteins are amino acids
...
A polypeptide is formed
when more than two amino acids join together
...
Amino acids all have the same structure
Polypeptide Formation
Amino acids are linked together by condensation reaction to
form polypeptides
...
The bond formed is called a peptide bond
...
The hydrogen on the –NH has an overall positive
charge and the O in the –C=O has an overall negative charge
...
Which makes it coil
into an alpha helix or beta pleated sheet
...
Weaker than disulfide and easily broken by changes in pH
...
The quaternary
structure is the way these chains are assembled
...
Test for Proteins – Biuret Test
As the test solution needs to be alkaline you first add sodium
hydroxide solution to the sample, then add copper sulfate
Title: Proteins
Description: All about the structure of proteins from amino acids to polypeptides, how they all come together and the tests.
Description: All about the structure of proteins from amino acids to polypeptides, how they all come together and the tests.