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Title: Microbiology- Identification of Unknown Bacteria - E.coli
Description: Performed different biochemical tests with pictures
Description: Performed different biochemical tests with pictures
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METABOLIC ACTIVITIES OF
MICROORGANISM – SCA & CUA
Simmons citrate agar/ Citrate
Utilization test
It is used for the differentiation of
Gram-negative bacteria on the
basis of citrate utilization
contains
sodium citrate
ammonium dihydrogen phosphate
pH indicator bromthymol blue
tests the ability of organisms to
utilize citrate as a carbon source
+ growth = green – blue
- growth = no color change
Christensen’s Urea Agar
Slant/Urea Hydrolysis
This test is done to determine a
bacteria’s ability to hydrolyze
urea to make ammonia using the
enzyme urease
...
The enzyme urease will be used
to hydrolyze urea to
make ammonia
...
Citrate and Urea Utilization of
the test bacteria
Bacteria
E
...
aerogenes
P
...
hardydiagnostics
...
htm
http://www
...
edu/microbugz/citrate_test
...
austincc
...
php
http://www
...
com/biochemical-testand-identification-of-e-coli/
http://www
...
com/oxidase-testprinciple-uses-procedure-types-resultinterpretation-examples-and-limitations/
https://catalog
...
com/cp_prod/Cont
ent/hugo/SIMMedium
Title: Microbiology- Identification of Unknown Bacteria - E.coli
Description: Performed different biochemical tests with pictures
Description: Performed different biochemical tests with pictures