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Biochemistry: Exploring the Molecular Basis of Life£5.00

Title: Antibiotic Resistance, Bacteria, Bacteriophages, Genetics, Microbiology
Description: The notes are centred around the antibiotic resistance topic, and where external information was mentioned, referencing took place. A lot of general knowledge University information is mentioned in the document, but for safety reasons, referencing had to be added.

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Study Notes – Antibiotic Resistance
I)

Background

Antibiotic resistance represents an evolutionary response of bacteria against antibiotics, by means
of an induced natural selection
...

The legal restriction of antibiotic consumption is an important solution to the antibiotic resistance
crisis
...

Conjugation (sexual transmission) and transduction (asexual transmission) represent two ways of
horizontal gene transfer
...
Bacteria with antibiotic resistance (R) plasmids represent the ones that spread ABR genes to
others
...


III)

Examples, their history and transformation

Examples of antibiotic resistant bacteria: Methicillin Resistant Staphylococcus aureus, Vancomycin
Resistant Enterococcus spp, Clostridium difficile, Extended Spectrum Beta-Lactamase, Acinetobacter
baumannii, Amp C and CDO
...

Before, most Staphylococcus aureus species were susceptible to antibiotics, such as Penicillin and
Methicillin
...

Enterococcus spp is a natural coloniser of human and animal digestive tracts
...


Clostridium difficile represents a Gram-negative, spore forming rod that is a strict anaerobe
...

Some Clostridium difficile strains produce toxins A (enterotoxic) and B (cytotoxic), and those strains
cause CDI, an infection in the digestive system
...

It is part of the ‘ESKAPE’ programme
...


IV)

Bacteriophages

They represent viruses that can be used to neutralise certain bacteria without harming the human
body, this process takes place through transduction
...

Reference list:


University of Essex, Colchester Campus, Life Sciences Department (Dr Aurélie Villidieu)



Frontiers (Article published by Nicola Principi, Ettore Silvestri and Susanna Esposito on 08/05/2019), Advantages
and Limitations of Bacteriophages for the Treatment of Bacterial Infections, Available at:
https://www
...
org/articles/10
...
2019
...


2) List three important antibiotic resistant bacteria and give the definition of two of them, by
choice
...
Explain your reasoning
...


6)
a)
b)
c)
d)

What sort of bacteria spread antibiotic resistance genes?
Bacteria with the F- plasmid (recipient only)
Bacteria with the F+ plasmid (donor only)
Bacteria with the R plasmid (both donor and recipient)
Bacteria with the HFr plasmid (both donor and recipient)

At the multiple choice questions, only one answer is correct
Title: Antibiotic Resistance, Bacteria, Bacteriophages, Genetics, Microbiology
Description: The notes are centred around the antibiotic resistance topic, and where external information was mentioned, referencing took place. A lot of general knowledge University information is mentioned in the document, but for safety reasons, referencing had to be added.