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CHEM 1E Experiment 5: Flame Tests and Absorption Spectroscopy Simulation
and Video Lab
This is a video and simulation experience to substitute for the CHEM 1E
Experiment 5
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You will fill in the provided data sheet and
upload into CANVAS LAB Section
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Spectroscopy can be used to identify elemental or molecular presence, or
to determine the concentration of molecular or atomic species in a system of interest
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Two types of
“spectra” most often obtained are emission and adsorption
spectra
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Some elements produce
very intense spectral lines that can be used to identify the
presence of the element
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In a flame test, a granule of an
ionic solid or a drop of a solution containing the dissolved ionic
solid is placed in a hot flame
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In absorption spectroscopy, the wavelengths of light absorbed by a
sample can be used to provide information about the presence of a species and the concentration
of the species
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The CuSO4 solution
used in this experiment has a blue color
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A higher concentration of the colored solution absorbs
more light (and transmits less) than a solution of lower concentration
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Each solution is transferred to a small, rectangular cuvette that is placed into the spectrometer
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When you graph absorbance vs
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The direct relationship between absorbance and
concentration for a solution is known as Beer’s law
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By locating the absorbance of the unknown on the vertical axis of the graph, the
corresponding concentration can be found on the horizontal axis
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OBJECTIVES
In this experiment, you will
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Observe flame tests on a variety of cation solutions and record observations
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Utilize you observations from standard flame test experiments to identify the
ion present in an unknown
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• Prepare a Beer’s law calibration curve from the test results of the standard solutions
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• Calculate the molar concentration of the unknown CuSO4 solution
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1
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2
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Table 1
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Screenshot of Beer’s Law Lab simulation immediately after loading
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Concentration is changed by sliding the
blue bar along the gradient
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Press the play button to start the simulation and select “Beer’s Law” on the right-hand
side (double click)
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Familiarize yourself with the controls
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Select CuSO4 as the solution, set
the wavelength to 780nm, and the meter to absorbance
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2
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Concentration CuSO4
Absorbance
0
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032M
0
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080M
0
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Construct a Beer’s Law Calibration curve using the values in Table 2
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Make sure to include title, axis titles, trendline, and display the
trendline equation on the graph
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)
4
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50A
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docx
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