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BIOCHEMICAL TECHNIQUES
1
...
Radio Immune Assay (RIA):
a) Is used for purification of hormones
b) Involves the use of radioactive elements
c) Is used for quantitative measurements of
hormones
d) Requires a pure antigen of known
concentration
e) "A" is the only wrong statement
3
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The following technique is the most suitable for the
separation of a mixture of the 20 amino acids:
a) Two dimensional electrophoresis
b) Gel filteration
c) Affinity chromatography

d) Dialysis
e) RIA
5
...
Dialysis:
a) Involves separation of molecules according to
density
b) Is based on diffusion across a porous
membrane
c) Is a type of chromatography
d) Can be used to separate 2 molecules with the
same molecular weight
e) The molecular weight is not important in the
technique

7
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Electrophoresis:
a) Is based on centrifugal force
b) Cannot be used for separation of proteins and
DNA
c) Requires electrical power source
d) Is based on passive diffusion of biomolecules
e) Is a technique for mammalian cell culture
9
...
In electrophoresis, the rate of migration depends
on:
a) Net electrical charge of the molecule
b) Size and shape of the molecule
c) Temperature of operation
d) Both A and B are correct
e) A, B and C are correct
11
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The following method is used only to detect RNA
and can be used to measure the difference in levels of
specific mRNA in different tissues:
a) Northern blot analysis
b) Polymerase chain reaction
c) Restriction fragment length polymorphism
linkage analysis
d) DNA fingerprint analysis
e) Southern blot analysis
13
...
Dialysis:
a) Is an ideal separation technique
b) Involves movement of molecules through a
porous membrane
c) Depends on the difference in size and
concentration
d) Is useful after affinity chromatography
e) B, C and D are correct
15
...
The following is correct:
a) Western blotting is for proteins
b) Northern blotting is for RNA
c) Southern blotting is for DNA
d) Transfer follows separation by electrophoresis
e) All of the above is correct
17
...
RFLP stands for:
a) Restriction fragment length position
b) Restriction fragment length polyploidy
c) Restriction fragment length phenotype
d) Restriction fragment length polymorphism
e) Restitution fragment loose polymorphism
19
...
The following techniques are used to immobilize
the corresponding biomolecules:
a) Southern blotting for proteins
b) Northern blotting for RNA
c) Southern blotting for DNA
d) Northern blotting for DNA
e) Both B and C are correct



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