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Why HPLC and GC are important and how you will differentiate between
them?
HPLC and GC are important why?
High performance liquid chromatography is an analytical technique which
is used for the separation and analysing the compounds via the mass
transfer of analytes (the substance which is analysed for mass
spectrometer) among the stationary and the mobile phases
...
This technique is widely used in the
analytical chemistry for the separation of compounds and have great
importance in the biochemistry and in industries for various purposes like
as the identifying, quantifying and also purifying the specific particles in the
mixture
...
So, it is very important
because it is used in chemical, medical labs as well as in pharmaceuticals
and also in other industries
...
Gaseous elements are analysed that are attach with the surface
of column that is actually cover with stationary phase
...
This type is mostly used in the chemistry labs
and other industries for the analysing of the specific oils, food products
and air pollutants
...
This type of chromatography is widely used in the industries for
the analysis of foods, drugs and also of the agrochemicals
...
The components that are
charges, polarity with molecular weight attach in the stationary
phase on the basis of their properties
...

• Normal phase separate the sample on the basis of adsorption
to the stationary surface polarity and this phase consists of
the polar stationary and non-polar mobile phase for the
separation of sample quickly
...

• Reverse phase contain the non-polar stationary phase and
polar mobile phase for the separation of sample quickly
...

Explanation about HPLC
Mobile phase is an important part of the machine because it is
involved in the segregation process
...
It is involve in the
advocating the ionization of analytes
...

Another part of the HPLC is the pump that govern pressure to the
liquid through the liquid chromatograph at the proper flow rate
(ml/min)
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Injector abstract the analytes into the flow stream of the mobile
phase and it play a role in govern the sample into the solvent stream
movement through the column
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Detectors are used in the machine
that detect the compounds that come from the column in which UV
and conductivity detectors are include
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Data system is basically a computer that control and maintain all the
parts of the HPLC that draw the signals from the detector and
measure the retention time of the sample qualitatively as well as
quantitatively
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HPLC can detect and identify the purity of
the compounds
...
it is widely used in the industries for
identifying the impurities
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➢ Principle
The principle of this process is occur in to the liquid stationary phase
and gas phase on the basis of participating quantity among the two
phases
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➢ Instruments
Carrier gas, separation column, detector, injector, thermostat/ ovens
and recorder are the main parts of the gas chromatography
...
Nitrogen, He, and
argon gases are mostly used in this machine’ cylinder
...
Hydrogen
may be used but the reaction can be held with unsaturated
compounds
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And the gaseous sample are inject via the pass loop and
with valves
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In which the
separation process is unique and there is no adsorption of polar
compounds to be held
...
Detectors is also an important part that
detect the signals of separated compounds and gave instructions to
the observer in which electron detector, photoionization detector
include
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In the food industries this
technique is usually used for the analysis of quality wise and also of
quantitatively and it have a great importance to identify
contaminants like as air pollutants, pesticides and other type’s
toxins
...
europeanpharmaceuticalreview
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chem
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edu/NEW/HPLC_Book/Rev
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html
• http://ctshplc
...
com/archives/2012/08/07/24851399
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com/gas-chromatography/#parts-ofgas-chromatography
• Instrumental Analysis by Gurdeep R Chatwal



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