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Describe the importance of microscopy and how we observe live cells in
microscopes?
Microscopy
It is the technical field which is used for examined the small objects that are
so small which cannot be seen by the naked eye
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Antonie Van Leeuwenhoek is
called as the father of the microscopes who made about five hundred
microscopes, he observe the drop of pond’s water and saw several small
objects he called them the animalcules
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which are used in
many fields of life to observe the tiny objects (micro-organisms)
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The objects which are very small which
can-not be seen by our eyes then these are objects observe via the various
types of microscopes
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It is also used commonly by the
jewellers to view the small objects of the jewellery
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It is also used in
cloth industry to check the fibres and also threads of the clothes
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Fleming in 1879, photosynthesis and cellular respiration in
1770 and Kerbs’ cycle in 1937 are observed with the help of microscopy
techniques
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Various types of microscopes are used to view the cells which
are too small that cannot be seen by naked eyes
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It is
mostly used in sciences labs for various purposes, like as to view the small
specimen’s bacteria, insects, and arthropods’ structures
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It have great

importance to analyse the forensic evidence, crime scenes like as layers or
bands of the bullets
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It plays an important
role in the genetics filed to analyse the genetic compositions and also
observe the abnormalities
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Firstly developed the simple microscope
but with the passage of time a lot of development came in the history of
world, now the electron microscope and probe microscope are highly used
because of its great effectiveness so, we can observe the internal structure
of the cells like as various types of organelles like as endoplasmic reticulum,
mitochondria and ribosomes etc
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Now the various blood samples, serum and urine samples can be
observe by the microscopy techniques
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Observance of live cells in the microscopes
In biomedical researches, cell biology and in pharmacology laboratories is a
vital aspect to observe the live cells and tissues
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After this
discovery the observance of living cells is so difficult then at last phase
contrast microscopy was develop to observe the un-stained living cells in
detail
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Frits Zernike developed
this technique and awarded by the Noble prize
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Its condenser have an
annular stopper with a thin transparent ring that create the cone of light
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Diverted
light is focus and form the clear image of the specimen
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Background is bright but the unstained
specimens look dark
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Then the phase contrast microscopy is
developed into Hoffman modulation and differential contrast microscopy
on the same principle
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The inverted microscope is very helpful to realize the living cells, it contain
condenser lens, light source, nosepiece, florescent illuminator and objective
lenses
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The cells are alive
in this microscope during the analysing
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It uses the rays of light for focusing the cells and the image is
form that can be seen by the objective lens
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Now the cells can be seen and analyse at the lower part of
the cell culturing apparatus via the ibidi polymer coverslip
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Inverted microscope
some live cells imaging
References
• https://en
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wikipedia
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m
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org/wiki/Microscopy#:~:text=Microscopy%20i
s%20the%20technical%20field,range%20of%20the%20normal%20ey
• https://sciencing
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html
• https://en
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wikipedia
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m
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org/wiki/Inverted_microscope



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