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CH-112L Lab 8 - Report
Name __Martha Wyne____________
Date __6/3/2022____________________
Partner(s) ____________________________________________________
Part A: Model of a Nucleotide
In the box below draw the complete Lewis structure (showing all atoms, bonds, lone
pairs, and charges) of the nucleotide built in video; a line-angle structure is not an
acceptable substitute
...
Q1
...
Which base is incorporated in this nucleotide?
Adenine
b
...
What is the name of this nucleotide? See Table 1 in the directions for possible
names
...
Describe the extracted DNA
...
Q4
...
Part C: Transcription and Translation
Part C1: Unmutated DNA
Data Table C1: Transcription and Translation of an Unmutated DNA Strand
Codon #
Coding
DNA
strand
(5’ 3’)
1
2
3
4
5
6
7
8
TTA
TCT
CAA
CGT
CCC
TTG
GGT
AAA
2
Template
DNA
strand
(3’ 5’)
AAT AGA GTT GCA GGG AAC CCA
TTT
mRNA
strand
(5’ 3’)
UUA
UCU
CAA
CGU
CCC
UUG
GGU
AAA
Amino
acid
sequence
Leu
Ser
Gln
Arg
Pro
Leu
Gly
Lys
Part C2: Mutated DNA
Q5
...
Substitution:
It is type of mutation where one base pair is replaced by a different base pair
...
Frameshift deletion:
It is a genetic mutation caused by removal or lose of intermediate section of
chromosome during reorganizing of nuclei
...
Data Table C2: Transcription and Translation of a Mutated DNA Strand
Codon #
Coding
DNA
strand
(5’ 3’)
Template
DNA
strand
(3’ 5’)
mRNA
strand
(5’ 3’)
1
TTA
2
TCT
3
4
5
6
7
8
GAA
CGT
CCA
TAG
GGT
AAA
AAT AGA CTT GCA GGT ATC CCA
TTT
UUA
AAA
UCU
GAA
CGU
3
CCA
UAG
GGU
Amino
acid
sequence
Leu
Ser
Glu
Ala
Pro
STOP
Gly
Lys
Mutated
codon?
No
No
Yes
Yes
Yes
Yes
No
No
Circle, box, or underline the location(s) in the template DNA strand of any
mutation(s) in the above table
Q6
...
g
...
The ribosome of polypeptide will read a different mRNA sequence resulting to
formation of a growing polypeptide chain hence the polypeptide is not shortened
...
4