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DATE: 17/08/2022
EXPERIMENT NO: 1
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QUADRANT STREAKING

AIM:
To perform quadrant streak method for isolation of pure cultures
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The resulting diminution of the
following inoculation, individual cells will be sufficiently for on apart on the surface of
the agar medium to separate the different species
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The steel plate is a rapid
qualitative isolation method
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The sample or the inoculum is diluted boo streaking it surface of agar plates
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When these lone bacterial cells divide and give rise to thousands and thousands
of new bacterial cells, an isolated colony is formed
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MATERIALS REQUIRED:
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A source of bacterial ( Stock culture, previously streaked agar plate or any
other inoculum )
Inoculation loop
Bunsen burner
Nutrient agar
Petri plates
Conical flask
Distilled water
70% Ethanol

PROCEDURE:
1
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The loop was
flamed and cooled it by touching an unused part of agar surface closed to the
periphery of the plates, and then dragged it rapidly several times across the
surface of areal
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The loop was reframed and cooled, and turned the petri dish 90°
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The loop should never enter the areal again
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The loop was reframed and cooled again and turned the petri dish 90°
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4
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Across area4, using a wider streak
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The flaming of the loops at
the points indicated is to dilute the culture so that fewer organisms are streaked
in each area

OBSERVATION:
Discrete colonies were observed
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DISCUSSION:
In a sterile work environment, using a sterile inoculation loop, streak were made in all
the four quadrants of the agar plate
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When observed after the incubation time isolated colonies were observed
in these plates
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Discrete colonies



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