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Title: Dialysis Lab
Description: A lab that uses a synthetic membrane to act like the cell's membrane when observing the animal cell.
Description: A lab that uses a synthetic membrane to act like the cell's membrane when observing the animal cell.
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Dialyzing solutions via synthetic membrane
John G
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In this lab, semi-permeable dialysis tubing is used to imitate the membrane of a cell
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PROBLEM : What chemicals can pass through semi-permeable dialysis tubing?
HYPOTHESIS: If the semi permeable dialysis tubing is replicating a biological membrane, then
the particles smaller in size will filter out correspondingly to the bigger particles, because of the
change in polarity as well as particle size
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Cut a 10 cm length of dialysis tubing 2
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Tie one end with string (tightly, no leaking) 4
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Tie the other end with string (tightly, no leaking) 6
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Weigh contents of tubing 8
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Do a
positive control: Conduct each of the four tests on the back with both water and the premix
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10
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Test
contents inside of tubing (see tests below) 12
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Did protein leave the inside of the membrane and go into the beaker? How did you prove this?
Since protein is a much more complex molecule with many different formulas varying many
different proteins, protein is a big particle and cannot leave the cell membrane as a result it will
stay in the dialysis bag
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3
2
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This then
proves that salt didn’t leave the beaker
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Did starch leave the inside of the membrane and go into the beaker? How did you prove this?
For the starch test, we added 3 drops of Iodine to detect if starch was present in the beaker, the
starch bonds with the starch molecules to form a compound which then gives us a yellow
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Did Sugar leave the inside of the membrane and go into the beaker? How did you prove this?
We added 10 drops of Benedict's solution to test the presence of sugar and when sugar and
benedict's react with each other, an orange color is produced, however in the negative water,
sugar reacts with Benedicts to produce a clear blue solution
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5
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Use your results from this lab to explain/justify your answer
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In the phospholipid
bilayer of the cell membrane, the lipids bilayer has hydrophobic tails which block protein, starch
and salt from crossing the membrane
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6
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A selectively permeable membrane will allow only what it decides in and out
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Smaller particles such as oxygen can cross the membrane, because it has a smaller particle
size allowing it to cross the membrane
Title: Dialysis Lab
Description: A lab that uses a synthetic membrane to act like the cell's membrane when observing the animal cell.
Description: A lab that uses a synthetic membrane to act like the cell's membrane when observing the animal cell.