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Title: ORGANIC MOLECULES LAB
Description: A study of the cell, the fundamental unit of biological structure and function. An introductory section on bioenergetics and biochemistry lay the groundwork for the consideration of the cell through a modern interdisciplinary approach. The course examines the ultrastructure of the cell and of the most universal cell functions: transport, cellular respiration, photosynthesis, cellular reproduction, and protein synthesis. Lecture themes are illustrated in the laboratory. 3 hours lecture/2 hours laboratory

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Bio-131-55
Professor Bladon
10/12/2022

Organic Molecules Lab

Starch Test
Blue black is a positive test indicating the presence of starch
Substance
Water
Starch
Glucose
Sucrose
Onion Juice
Piece of Potato

Final Color
Orange/Yellow
Orange/Yellow
Orange/Yellow
Orange/Yellow
Orange/Yellow
Blue/Black

+ or - result
Negative Positive +
Negative Negative Positive +
Positive +

Benedicts Test for reducing sugars
Positive Benedict’s is a green-yellow-orange-red color
Substance
Water
Starch
Glucose
Fructose
Sucrose
Onion Juice
Piece of Potato

Final Color
Blue
Blue
Red/Orange
Red/Orange
Blue
Red/Orange/Green
Green

+ or - result
Negative Negative Positive ++++
Positive ++++
Negative Positive +++
Positive +

Hydrolysis of Sucrose
Tubes
Sucrose and Invertase
Sucrose and Water
Sudan IV Test for Lipid
Red droplets or a red layer is a positive for lipids

Color After Boiling
Yellow/Orange
Blue/Light Blue

Tubes
Water
Corn Oil
Sucrose

Observation
Light Pink
Light Pink, layer of fat over
water
Light Pink

+ or – for lipids
Negative Positive +

Final Color
Light Blue
Purple
Light Blue
Yellow/Green

+ or – result
Negative Positive +
Negative Negative -

Negative -

Biuret Test for Proteins
Positive Biuret test is purple
Substance
Water
Egg Albumin
Amino Acid
Onion Juice


Title: ORGANIC MOLECULES LAB
Description: A study of the cell, the fundamental unit of biological structure and function. An introductory section on bioenergetics and biochemistry lay the groundwork for the consideration of the cell through a modern interdisciplinary approach. The course examines the ultrastructure of the cell and of the most universal cell functions: transport, cellular respiration, photosynthesis, cellular reproduction, and protein synthesis. Lecture themes are illustrated in the laboratory. 3 hours lecture/2 hours laboratory