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AUTOCLAVE
Principle:
According to Boyle’s Law boiling point is directly proportional to the
pressure
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Hence if the pressure is 15lbs per
square inch, the corresponding temperature will be 121ºC
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Thereby rapidly heating the
objects on which it condenses
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Hence a temperature of 121ºC; 15lbs pressure of steam in an
autoclave effectively kills all the living organisms in about 15-20 min
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In case of spore bearers, hot
steam condenses on their relatively cooler surfaces
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Other
than microorganisms; media, filter paper discs, glass apparatus, water
samples, surgical instruments, injections, discarded material etc
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To check whether sterilization has been effective, place the media flask in
the incubator for 18-24 hrs at 37°C
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Procedure:
1
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Next, cover the cotton plugs with clean, rough brown paper,
tying it in place with twining thread or covering it with aluminium foil
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2
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3
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Place the filled container inside the autoclave with caution
now
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Place the lid on top of the container and secure it using the given knobs,
fastening the opposite knobs at the same time
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After turning on the power connection, loosen the steam release
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To make sure all the
air has been released, watch for bubbles emerging and shut off the steam
release
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At this point, steam begins to build up inside the chamber and the
pressure gauge needle begins to rise
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7
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Safety:
When using an autoclave, always wear personal protection equipment
(PPE)
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To protect your arms from heat and steam burns, wear longer heatresistant gloves and a lab coat
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Throughout a cycle, avoid
opening the autoclave
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