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NOTES
INTRODUCTION OF BLOOD

INTRODUCTION
IMPORTANT UNIT IN HOSPITAL
 OBJECTIVE: TRANSFUSION BLOOD CENTER
AND COLLECTION NEEDS FOR CLINICAL
 OPERATE 24 HOURS & CONTROLLED BY
MLT
 TASK MLT: CHOOSE THE BLOOD / BLOOD
COMPONENTS SUITABLE FOR
TRANSFUSED REFERS TO CASES TO
PATIENTS


SERVICES PROVIDED IN THE
LABORATORY OF THE BLOOD
BLOOD COLLECTION
 PROCESSING & SEPARATION OF BLOOD
COMPONENTS
 CLASSIFICATION OF BLOOD GROUP
 SCREENING & CERTIFICATION Ag-Ab CASE
FOR DISEASES (HIV, hep B and hep C) OTHER
BLOOD GROUP (Kell, Duffy, MNSs)
 COMPATIBILITY OF BLOOD TEST (GXM)
 GROUP, SCREEN & HOLD (GSH)
 Coomb's Test (IAT and DAT)


COLLECTION
TECHNIQUES OF BLOOD
APHERESIS MANUAL :
~ COLLECTION OF WHOLE BLOOD
~ SEPARATION OF COMPONENTS OF THE
MANUAL
 APHERESIS AUTOMATIC:
~ OTHER COMPONENTS IN THE BLOOD
COLLECTION (ADR, PLASMA)
~ SEPARATION OF COMPONENTS
AUTOMATIC


BLOOD DONATION
HEALTHY BODY
 AGE: 18 YEARS & OVER
 Hb NORMAL LEVEL : L = 13
...
5
 BODY WEIGHT: OVER 99 POUNDS
 BODY TEMPERATURE, BP, NADI : NORMAL
 Not suffering from any chronic diseases
 DO NOT TAKE ANY MEDICATIONS UNLESS
DOCTOR ON ORDER
 ENOUGH SLEEP


GROUPING BLOOD






SINGLE BAG( 300 ml & <45 kg ) :
~ WHOLE BLOOD(WB)
DOUBLE BAG( 450 ml & >50 kg ) :
~ LEUCOCYTE POOR WHOLE BLOOD(LPWB)
~ PLATLET CONCENTRATION(PC)
TRIPLE BAG( 450 ml & >50 kg ) :
~ LEUCOCYTE POOR PACKED CELL(LPPC)
~ PACKED CELL
~ PLATLET CONCENTRATION(PC)
~ CRYOSUPERNATAN
~ CRYOPRECIPITATE @ AHF
~ FRESH FROZEN PLASMA(FFP)

GROUPING BLOOD
( SINGLE BEG)
VOLUME OF BLOOD: 300 mL
...

 TYPE OF ANTICOAGULANT : CPDA
 PERIOD OF RETENTION : 35 DAYS


GROUPING BLOOD
(DOUBLE BAG)
VOLUME OF BLOOD : 450 mL
...
2°F)
~ PLATELET CONCENTRATION
(3 DAYS, 71
...



GROUPING BLOOD
(TRIPLE BAG)


VOLUME OF BLOOD : 450 mL
...
6°F
...

~ CRYOPRECIPITATE : 2 YEARS, -32
...

~ CRYOSUPERNATANT : 2 YEARS, -32
...


ANTICOAGULANT
CPD
OBJECTIVES

ANTICOAGULANT & BLOOD STORAGE

LIFESPAN

21 DAYS

MATERIAL

SODIUM CITRATE
ASID CITRIC
DEXTROSE
MONO BASIC SODIUM PHOSPHATE

ACTION

Undergraduate AVOID IN BLOOD
BALANCING OF BLOOD (pH)
NOURISHMENT SDM
ATP INCOME SUPPORT

ANTICOAGULANT
CPDA
OBJEKTIVES

ANTICOAGULANT & PENYIMPANAN DARAH

LIFESPAN

35 DAYS

MATERIAL

SODIUM CITRATE
ASID CITRIC
DEXTROSE
MONO BASIC SODIUM PHOSPHATE
ADENINE

ACTION

MENGELAKKAN KOAGULASI DALAM DARAH
MENGIMBANGI pH DARAH
SUMBER NUTRISI SDM
MENYOKONG PENGHASILAN ATP
MENGEKALKAN ARAS ATP DALAM SDM

ANTICOAGULANT
S
...
G
...
2F)

BLOOD PROCCESSING
(DOUBLE BAG)
DOUBLE BAG
(450 ML)

SOFT SPIN
A) CRYOFUGE : 2330 RPM, 8M, 71
...
6OC

PLATELET RICH PLASMA (PRP)

BLOOD PROCCESSING
(DOUBLE BAG)
PLATELET RICH PLASMA (PRP)

HARD SPIN
A) CRYOFUGE : 3660 RPM, 15M, 71
...
6OF

A) BUFFY COAT POOR WHOLE BLOOD(35
DAYS, 39
...
6OF)

BLOOD PROCCESSING
(TRIPLE BAG)
TRIPLE BAG
( 450 ML )

SOFT SPIN
A) CRYOFUGE : 2330 RPM, 71
...
6F

A) SAGM + BUFFY COAT POOR PACKED CELL
(42 DAYS, 39
...
6OC
B) BECKMAN : 4000 RPM, 10M, 71
...
6OF)
B) FFP (2 YEARS, -94F)

PEMPROSESAN DARAH (TRIPLE
BAG)
FFP

TEST SCREENING

STORAGE AT TEMPERATURE -96
...
2OF
B) BECKMAN : 4000 RPM, 10M, 39
...
A
...
M + PACKED CELL
(42 DAYS, 39
...
2OF)

SPIN : 3660 RPM, 30 M, 39
...
8OF)
B) CRYOSUPERNATAN (2 YEARS, -96
...
8OF

FUNCTION

SUPPLY ALL THE ABOVE

CASE

THERAPY CASE von Willebrand & HEMOPHILIA A
DUE TO CONTROL BLEEDING
fibrinogen deficiency
Factor XIII Deficiency RAWAT

BLOOD COMPONENT
PLATELET
CONTENT
LIFESPAN

PLATELET IN 40-70 ML PLASMA
5 DAYS, 71
...
8OF

FUNCTION

IMPORTANT CASES HEMOSTASIS

CASE

CASE FOR THE TREATMENT PLATELET
ABNORMALITIES
THROMBOCYTOPENIA ( <2 X 109/L )
LEUKEMIA AKUT,NEOPLASIA SUM-SUM
BONES ( PLATLET < 10 000/µl )
DENGUE

BLOOD COMPONENT
LEUKOSIT / GRANULOSIT COMPONENT
CONTENT

Leukocytes & PLATELET

LIFESPAN

FUNCTION

MIGRATION, FAGOSITOSIS & DESTROY
BACTERIA IN BLOOD

CASE

NEUTROPENIA ( <500/µl )
Pt TERIMUNOKOMPROMI
INFECTION
IF ANTIBIOTICS NOT EFFECTIVE

THANK YOU !!



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