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DNA Replication
Created
Class
@January 20, 2025 3:36 PM
Cells to Systems
Edwin Chargaff Rules
Amounts of A-T and G-C are equal but amounts differ between species
Nucleic acid are polymers
Monomers= Nucleotides
Base +sugar + phosphate= Nucleotides
Base can be made of Purine or Pyrimidine
Examples of Purines= Adenine/ guanine
Examples of Pyrimidine= Thymine, uracil, cytosine
Sugar can be Deoxyribose or ribose
DNA Synthesis
New DNA is synthesized from dNTPs
During replication, 3’ OH group of last nucleotide attacks 5’ phosphate
group of incoming dNTP
Phosphates are cut off
Two nucleotides form a phosphodiester
This causes phosphate ions to be released
DNA Replication
DNA Replication
1
Perfect replication until mitosis
Requires primer, template, enzymes, dNTPs, ribonucleotide triphosphates
Enzymes
RNA polymerase
DNA polymerase
Adds nucleotides to growing polymer
DNA ligase
Catalyses formation of phosphodiester bond between 3’ Hydroxyl and
5’ Phosphate groups in d