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Experiment 5: Viscosity of Solutions: Molecular Weight of Dextran
I
...
of viscometer
14
...
27
...
359
Sample conc (wt Average Efflux Viscosity
Specific
Run 1
dextran/vol)
Time, sec
viscosity, ɳsp
ɳsp/C
Distilled water
233
...
00840
0
N/A
03:53
...
0006g/100mL
293
...
01054
0
...
25451
04:53
...
5003g/100mL
264
...
009493
0
...
26009
04:24
...
2502g/100mL
255
...
009172
0
...
36736
04:14
...
1251g/100mL
243
...
008747
0
...
32990
04:02
...
0625g/100mL
238
...
008587
0
...
35670
03:59
...
012
0
...
008
0
...
004
0
...
53
...
80
04:23
...
83
04:04
...
61
ɳsp /C vs
...
1167x + 0
...
69752
0
...
0021x + 0
...
98784
ɳsp /C
Viscosity
Viscosity vs Concentration
1
...
3
0
...
1
0
0
0
...
4
0
...
8
1
Concentration (g/100mL)
1
...
2
0
...
6
0
...
2
Concentration
II
...
Water is a good solvent for dextran because it is able to expand chains of
polysaccharides in the dextran
...
This causes resistance in the flow of liquid, which then defines
the viscosity
...
8oC, according to the textbook, is 0
...
cm/sec
...
The viscosity was calculated by dividing the sample efflux time by the efflux time of water then
subtracting it by 1
...
The y-‐intercept (intrinsic viscosity of dextran) was determined to be 0
...
This value can be plotted against
molecular weight to get the molecular weight of the unknown
...
5000 while K is 0
...
Using
N=KMa, the molecular weight was calculated to be 134,744
...
III
...
Based on the results, as the concentration of dextran increased, the more it is viscous;
the viscosity of the sample increases as the concentration increases
...
359 and the molar mass was calculated to be 134,744
...
IV
...
00840x293
...
01054
Time of water
233
...
215/233
...
2546 ratio
3) ɳsp/C = 0
...
0006 = 0
...
359=0
...
5000 à M=(0
...
000978)2 = 134,744
...
Literature Cited
“Viscosity
...
Web
...
ac
...
pdf>