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Title: Gram Positive vs Gram Negative Revision
Description: A simple poster to aid your revision of Microbiology - Gram negative and positive bacteria.
Description: A simple poster to aid your revision of Microbiology - Gram negative and positive bacteria.
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Gram Positive
Gram Negative
Colour on Gram Stain
BLUE
PINK
Thickness of peptidoglycan layer
90% of cell wall, each cable is about
50nm wide
10% of cell wall
Outer membrane
NO
YES
Type of crosslinks in peptidoglycan Occurs through short peptide
interbridge
Formed by peptide bond from amino
group of DAP of glycan chain to
carboxyl group of terminal D-alanine
on adjacent glycan chain
Example of species
Staphylococcus aureus
Escherichia coli
Symptoms associated
Staphylococcal food poisoning,
presents with more prominent severe
vomiting than diarrhoea
...
Smear onto slide, stain with crystal
violet, add iodine
Smear onto slide, stain with crystal
violet, add iodine, wash with organic
solvent (counterstained with safranin)
How to Gram stain
Only cause diarrhoea
Title: Gram Positive vs Gram Negative Revision
Description: A simple poster to aid your revision of Microbiology - Gram negative and positive bacteria.
Description: A simple poster to aid your revision of Microbiology - Gram negative and positive bacteria.