Notesale: Turn your study into money

Document Preview

Extracts from the notes are below, to see the PDF you'll receive please use the links above

---

NadiaA

Introduction to Histology
Background
Histology- tissue biology, it looks at the morphology and relates this to the function
Understand what happens in a normal, healthy body and therefore understand what happens when
something wrong happens= disease
Regenerative medicine- a branch of research in tissue engineering and molecular biology which
deals with the "process of replacing, engineering or regenerating human cells, tissues or organs to
restore or establish normal function"
...
g
...
g
...

Formalin is usually used; 37% formaldehyde

Tissue sampling
Pale area removed is tumour
Take blocks of sample and embed into paraffin:





Tissue block is passed through graded alcohol to dehydrate it
Put into solvent, that is miscible with alcohol and paraffin wax
Tissues embedded into molten wax
Person can cut and section it

Sections need to be thin to be able to shine light through it to examine it and see the structures

1

NadiaA

Staining- Haematoxylin and eosin







Basic dyes e
...
haematoxylin have a positive charge and bind to negatively charged
components e
...
nuclear chromatin, cytoplasmic RNA, some extracellular matrix proteins
such as cartilage
...

Acidic dyes such as eosin have a negative charge and thus bind to positively charged
components of tissue e
...
cytoplasmic proteins such as cytoskeleton, intracellular
membranes, and most extracellular matrix protein fibres such as collagen
...

Basophilia= blue, if haematoxylin is used
Acidophilia- binding of acidic dye to tissue, pink if eosin is used

Microscopy
To see ultrastructure, an electron microscope is more suitable as it has higher resolution and
magnification

2



---