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EXPERIMENT NO: 1
DATE:
PREPARATION OF BUFFER
AIM:
To prepare the buffer at required pH
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For measurement of pH, an electrode system sensitive
to change in H+ ion concentration of solution is taken
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PROCEDURE:
1
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2M: 1
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Sodium Acetate Solution: 0
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72gm of sodium
acetate trihydrate is dissolved in 100ml Distilled water
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2ml of sodium acetate solution into 100ml of
standard flask and add 14
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This gives 0
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The pH is measured with pH meter
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Wash electrode with
distilled water and introduced into 0
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6
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2ml Sodium acetate and 14
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pH was measured initial reading observed was 4 which made
upto 4
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2
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• Sodium diethyl barbititrate
PROCEDURE:
Dissolve 2
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2gm of sodium diethyl
barbititrate in distilled water and upto 1 liter
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The pH meter is first standararised with pH buffer
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8
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CITRATE BUFFER:
REAGENT S REQUIRED:
• Citric acid: Dissolve 2
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• Sodium citrate solution 0
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941gm of sodium citrate in
100ml distilled water
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5ml of citric acid with 3
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It corresponds to 0
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This gives citrate buffer at
pH 2
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RESULT:
Citrate buffer was prepared and the pH observed was 4
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5 using 1N Hcl and 5N NaoH
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CARBONATE- BICARBONATE BUFFER:
REAGENTS REQUIRED:
• Sodium carbonate solution 0
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12gm of anhydrous
sodium carbonate in 100ml Distilled water
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68gm of sodium bicarbonate in
100ml of distilled water
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5ml of sodium carbonate (Na2Co3) solution
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5ml of sodium bicarbonate solution and made upto 100ml with distilled
water which corresponds to 0
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Standardise pH meter and measure the pH of required buffer
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2
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5 which
was adjusted to 10
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5
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78gm of sodium dihydrogen phosphate in 100ml
of distilled water
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2M): Dissolve 5
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17 gm sodium hydrogen phosphate in 100ml distilled
water
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This gives
phosphate (Po4)2 buffer of 0
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Standardized pH meter with standard buffer
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The pH of the
solution is 6
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RESULT:
Phosphate buffer was prepared and pH was observed 8
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8 using 1N Hcl and 5N NaoH
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POTASSIUM PHOSPHATE BUFFER:
REAGENTS REQUIRED:
• Dipotassium hydrogen phosphate
• Potassium dihydrogen phosphate
PROCEDURE:
174
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09 g/mol potassium
dihydrogen phosphate was taken and made up to 200ml using distilled water
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Standardised pH meter with standard buffer
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The pH of the
solution is 6
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RESULT:
Dipotassium hydrogen phosphate (K2HPO4) and potassium dihydrogen
phosphate (KH2PO4) solution were prepared and the pH was measured to be 9
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23 respectively, the solution were made using 1N Hcl and 5N NaoH
respectively and the pH was found to be 6
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