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Exercise & Cellular Respiration
Purpose:
The purpose of this lab activity is to analyze the affect of exercise on cellular respiration
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Purpose
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 To identify the role of carbon dioxide production, breathing rate, and heart rate in determining
the rate of cellular respiration
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Background Information
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Cellular
respiration requires oxygen (which is breathed in) and creates carbon dioxide (which is breathed out)
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You will measure 3 different indicators of cellular respiration: breathing rate, heart rate,
and carbon dioxide production
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Breathing rate is measured in breaths per minute, heart rate in beats per
minute, and carbon dioxide in the time it takes bromthymol blue to change color
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BTB is an acid indicator; when it reacts with acid it turns from blue to
yellow
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The more carbon dioxide you breathe into the BTB solution, the faster it will change
color to yellow
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1
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2
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Write a prediction/hypothesis of how exercise will affect your body’s production of carbon dioxide
(i
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do you think your body will produce more or less carbon dioxide as you exercise)
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Procedure:
PART A: Resting (no exercise)

Measuring Carbon Dioxide Production:
1
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3
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5
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Use a graduated cylinder to measure out 20 mL of tap water and pour it into a small beaker
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Using a straw, exhale into the BTB solution
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Record the time in Table 1
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Average the results of the 3 trials
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Measuring Breathing Rate:

1
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Record this in
Table 2
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Repeat this 2 more times
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Average the 3 trials to get your average breathing rate
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Measuring Heart Rate:

1
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2
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Record this in Table 3
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Repeat this 2 more times
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Average the 3 trials to get your average heart rate
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PART B: Increased Muscle Activity (Exercise)
1
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2
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3
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Time
how long it takes for the BTB to turn yellow
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4
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You only need to do
this once
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Record these values in Tables 2 & 3
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6
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7
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Time how long it takes for the
BTB to turn yellow
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8
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You only need to do
this once
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Record these values in Tables 2 & 3
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If there is time, repeat the entire procedure for your lab partner
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Results:

Analysis & Conclusions: Answer the questions below using your BACKGROUND information in the lab,

as well as your lab data
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How did exercise affect the time needed for the solution to change color? Explain why the
color change occurred (How does BTB work?)
2
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What can you conclude about the effect of exercise on breathing rate? Why is this so?
4
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State whether your hypothesis was correct or incorrect and why
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Address the need to
get oxygen to the muscles and get rid of carbon dioxide, as well as how the muscles cells get
the energy needed to continue contracting

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