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Title: BIOCHEMICAL TESTS FOR BACTERIA
Description: An organized table for Bacteriology/Microbiology students of the different biochemical tests for bacteria including its principle, indicators, color changes and quality control.
Description: An organized table for Bacteriology/Microbiology students of the different biochemical tests for bacteria including its principle, indicators, color changes and quality control.
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TEST
CARBOHYDRATE FERMENATION
TEST
1
...
coli (-) K
...
coli (-) Klebsiella,
Enterobacter
(+) Escherichia coli
(-) Enterobacter cloacae
(+) Staphylococcus aureus
(-) Streptococcus pyogenes
(+) Enterobacter cloacae
(-) Escherichia coli
(+) Klebsiella pneumoniae
(-) Escherichia coli
(+) Neisseria gonorrhoeae
(-) Escherichia coli
ACIDIC – YELLOW
ALKALINE – RED
NEUTRAL - ORANGE
Tryptophan to pyruvic acid, ammonia
1
...
ERLICH’s’
PDAB – add paradimethylaminobenzaldehyde
PDAB add Xylene/Ether
QUALITY CONTROL
Determines whether gram-neg rod
utilizes glucose, lactose, sucrose
fermntatively and forms H2S
...
SPOT INDOLE
METHYL RED / VOGUES-PROSKAUER
TEST
1
...
BUFFERED PEPTONE
GLUCOSE BROTH
CATALASE TEST
VOGUES-PROSKAUER TEST
Barrit’s method for Gram (-)
1
...
SOLUTION B (KOH)
CITRATE TEST
1
...
PADAM Pink - Black
-para-aminodimethyl aniline
monohydrochloride
2
...
5 below
(-)…
...
5
(+) EFFERVESCENCE
(Bubble formation)
(-) Lack or weak bubble
formation
(+) PINK BURGUNDY layer
on top of the broth in 10
mins
(-) YELLOW
(+) BLUE – medium alkaline
(-) YELLOW – acid PH
(-) GREEN – neutral PH
DARK PURPLE – 10
seconds
TEST
UREA HYDROLYSIS
1
...
8 to
magenta at 8
...
1
(-) ORANGE – Acid pH 6
Title: BIOCHEMICAL TESTS FOR BACTERIA
Description: An organized table for Bacteriology/Microbiology students of the different biochemical tests for bacteria including its principle, indicators, color changes and quality control.
Description: An organized table for Bacteriology/Microbiology students of the different biochemical tests for bacteria including its principle, indicators, color changes and quality control.