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Title: IgG incubation: Pepsin Vs Papain
Description: About 75% of all immunoglobulins in our body is IgG. IgG is small and light weighing ( about 146 kilodaltons) with gamma heavy chain. The molecule is 'Y' shaped and has fab ( fragment antigen binding ) and fc ( fragment crystalline ) regions. Between fab and fc is the hinge region. Papain the enzyme isolated from pappaya tree latex splits the IgG molecule into 3 equal pieces by cleaving the hinge region- 2 fab pieces and 1 fc. Papain digested antibody is unable to promote agglutination and lysis. Whereas pepsin the enzyme isolated from stomach juice digest the IgG molecule and yields 1 (fab)2 molecules and 1 fc molecule.
Description: About 75% of all immunoglobulins in our body is IgG. IgG is small and light weighing ( about 146 kilodaltons) with gamma heavy chain. The molecule is 'Y' shaped and has fab ( fragment antigen binding ) and fc ( fragment crystalline ) regions. Between fab and fc is the hinge region. Papain the enzyme isolated from pappaya tree latex splits the IgG molecule into 3 equal pieces by cleaving the hinge region- 2 fab pieces and 1 fc. Papain digested antibody is unable to promote agglutination and lysis. Whereas pepsin the enzyme isolated from stomach juice digest the IgG molecule and yields 1 (fab)2 molecules and 1 fc molecule.
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Title: IgG incubation: Pepsin Vs Papain
Description: About 75% of all immunoglobulins in our body is IgG. IgG is small and light weighing ( about 146 kilodaltons) with gamma heavy chain. The molecule is 'Y' shaped and has fab ( fragment antigen binding ) and fc ( fragment crystalline ) regions. Between fab and fc is the hinge region. Papain the enzyme isolated from pappaya tree latex splits the IgG molecule into 3 equal pieces by cleaving the hinge region- 2 fab pieces and 1 fc. Papain digested antibody is unable to promote agglutination and lysis. Whereas pepsin the enzyme isolated from stomach juice digest the IgG molecule and yields 1 (fab)2 molecules and 1 fc molecule.
Description: About 75% of all immunoglobulins in our body is IgG. IgG is small and light weighing ( about 146 kilodaltons) with gamma heavy chain. The molecule is 'Y' shaped and has fab ( fragment antigen binding ) and fc ( fragment crystalline ) regions. Between fab and fc is the hinge region. Papain the enzyme isolated from pappaya tree latex splits the IgG molecule into 3 equal pieces by cleaving the hinge region- 2 fab pieces and 1 fc. Papain digested antibody is unable to promote agglutination and lysis. Whereas pepsin the enzyme isolated from stomach juice digest the IgG molecule and yields 1 (fab)2 molecules and 1 fc molecule.