Search for notes by fellow students, in your own course and all over the country.
Browse our notes for titles which look like what you need, you can preview any of the notes via a sample of the contents. After you're happy these are the notes you're after simply pop them into your shopping cart.
Title: Cryobiology and cryopreservation
Description: Introduction and application to the science of cryobiology, including uses in conservation and medicine. Differentiation between cryobiology and cryopreservation and consideration of cold-induced injuries on the viability of preserved tissue. Notes were taken from 2nd year undergraduate Microbiology but also very useful in fields such as Medicine, Conservation Biology and Zoology. Lecture given by Professor Tiantian Zhang of Bournemouth University, who is one of the leading world experts in cryopreservation.
Description: Introduction and application to the science of cryobiology, including uses in conservation and medicine. Differentiation between cryobiology and cryopreservation and consideration of cold-induced injuries on the viability of preserved tissue. Notes were taken from 2nd year undergraduate Microbiology but also very useful in fields such as Medicine, Conservation Biology and Zoology. Lecture given by Professor Tiantian Zhang of Bournemouth University, who is one of the leading world experts in cryopreservation.
Document Preview
Extracts from the notes are below, to see the PDF you'll receive please use the links above
Cryobiology and cryopreservation
-
-
-
-
Cryobiology – study of life at low temperatures
Cryopreservation of cells, tissues, gametes, embryos
Preservation of organs under hypothermic conditions for transplantation
Cryopreservation – technique by which viable cells, tissues, organs, even organisms
can be sustained at a low temperature
o Liquid nitrogen at -196°C
Required that changes in genetic material is prevented, patterns of gene expression
reliably reproduced in recovered material
Cell suspensions and thin tissue sections can be stored almost indefinitely in liquid
nitrogen
Large organs are usually stored and transported for short times at cool but not
freezing temperatures
Cryopreserved human sperm, eggs and embryos routinely used in infertility
treatments
Cryopreservation of gametes and embryos of – cattle, sheep, goats, pigs, birds,
reptiles, amphibians, fish – conservation
Cryobanks – cryopreservation of gametes and embryos of endangered animal
species
...
Freezing injury – solidification of ice destructive and even lethal to cells (Intracellular
ice)
o Ice formation occurs in liquid solutions first
...
g
...
High molecular
weight, cannot permeate into cells; protect cell membrane
o Reduce concentration of damaging solutes
o Increase unfrozen fraction
o Reduce volume deviation
o Permeating Cryoprotectants protect cells from slow freezing injury
o Cryoprotective effect of non-permeating Cryoprotectants mainly based on
cell dehydration and reduce ice crystal formation
Controlled slow cooling (glassy state) – water from liquid to solid without ice crystal
formation
Vitrification – preservation at extremely low temperature without ice freezing
o Formation of glassy or solid state – no ice crystals
o Relies of high concentration of solutes (typically a mixture of
Cryoprotectants) and extremely rapid cooling of sample
o Has been applied ton preservation of tissues, blood cells, D
Title: Cryobiology and cryopreservation
Description: Introduction and application to the science of cryobiology, including uses in conservation and medicine. Differentiation between cryobiology and cryopreservation and consideration of cold-induced injuries on the viability of preserved tissue. Notes were taken from 2nd year undergraduate Microbiology but also very useful in fields such as Medicine, Conservation Biology and Zoology. Lecture given by Professor Tiantian Zhang of Bournemouth University, who is one of the leading world experts in cryopreservation.
Description: Introduction and application to the science of cryobiology, including uses in conservation and medicine. Differentiation between cryobiology and cryopreservation and consideration of cold-induced injuries on the viability of preserved tissue. Notes were taken from 2nd year undergraduate Microbiology but also very useful in fields such as Medicine, Conservation Biology and Zoology. Lecture given by Professor Tiantian Zhang of Bournemouth University, who is one of the leading world experts in cryopreservation.