Notesale: Turn your study into money

Document Preview

Extracts from the notes are below, to see the PDF you'll receive please use the links above

---

L7 Endothelium




Lines all blood and lymphatic vessels
Majority of endothelial cells are present within microvasculature (arterioles, capillaries, venules)
The velocity of the blood affects cell morphology

Human umbilical vein endothelial cells allowed the study of endothelial function in isolation:
 Migration, permeability, proliferation, survival, angiogenesis (tube formation and sprouting assays in
extracellular matrix gels)
Developmental origin of ECs
 Haemangioblast  Haemogenic Endothelium  Definitive Haematopoietic Progenitors
 Markers of endothelial & haematopoietic precursors can be used to define stages of cell differentiation
 Blood islands  fusion  primary capillary plexus  Expand and remodel  whole vascular system and then
lymphatic system
Maturation of Vessels - PICTURE
Pericyte– like smooth muscle cells
Lymphatic vessels have loose tight junctions – are porous
Phenotypic Heterogeneity
 Allows endothelium to conform to the diverse needs of underlying tissues throughout the body
 Allows adaptation to diverse microenvironments e
...
hypoxic hyperosmotic environment of the inner medulla of
the kidney vs
...
g
...
Inhibition of VEGF 
hypertension, proteinuria, bleeding gums
o VEGF and vascular permeability
 VEGF was originally discovered as “vascular permeability factor (VPF)” based on its ability to
induce permeability (miles assay)
 Systemic delivery of VEGF induces increased vascular permeability to fluids and solutes by
inducing, fenestrations & loosening of adherens junctions between EC
...
g

---