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Title: Laboratory legislation and safe working practices
Description: This document contains definitions as well as detailed information on the governing bodies control laboratories, risk assessments, hazard grouping, laboratory containment levels, microbiological safety cabinets (MSC) 1 - 3, legislation on genetically modified organisms
Description: This document contains definitions as well as detailed information on the governing bodies control laboratories, risk assessments, hazard grouping, laboratory containment levels, microbiological safety cabinets (MSC) 1 - 3, legislation on genetically modified organisms
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TW2 – 3rd October – Safe Working Practices
Definitions
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HEPA: High Efficiency Particulate Air (filter) usually sit at the top of MSC cabinets
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Current legislation
Control of Substance Hazardous to Health (COSHH)
Genetic modified organism (GMO) – regulation 2000
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Government bodies
Health and Safety Executive (HSE) Act 1974
Advisory Committee on Dangerous Pathogens (ACDP) 1981 (x3 meetings p/ann)
The National Counter Terrorism Security Office (NaCTSO)
Biological Safety Office (BSO) safety committee
...
coli 0157, clostridium botulinum and tetani
Fungi: Candida albicans (thrush)
Virus: Epstein-barr (glandular fever), BCG vaccine, polio
Parasites: Toxoplasma gondii
Group 3 – cabinet – can’t be sent through post
Can cause serve human disease
May spread throughout the community
Treatment still readily available on the whole
Bacteria: Bacillus antracis, TB
Fungi: Histoplasma
Virus: HIV, rabies, hepatitis B
Parasite: Trypanosoma brucei rhodesiense (toxoplasmosis)
Group 4 – viruses only
Does cause serve human disease
Likely to spread throughout the community
Treatment mainly unavailable/ineffective
Workers never come directly into contact with pathogens
Biohazard suit must be worn, with materials in class 3 MSC
Can’t be posted, police escort, expert used to handling pathogen, government
approval before movement
Viruses: Ebola fever, smallpox virus, lassa fever
Relates directly to containment levels (1-1, 2-2 etc)
In exam question if it asks about categories, give specific examples of microbes that
you can and can’t handle
Laboratory containment levels
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C3 and 4 are always under negative pressure with extract filter through HEPA filter
(in cat 4 the air filter on input and double on extract air
C3 and 4 have observation window
All lab should have bench top that are resistant to acid, alkalis, solvents and
disinfectants
Microbiological safety cabinets (MSC) – MUM I
For containment level 2 and 3 if risk of aerosol exposure
Make and type must be correctly selected
Used correctly
Maintained correctly
Installed correctly
-
MSC 1, 2, 3 – Relates to how the cabinet operates and the protection it provides
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MSC maintenance – 6 monthly airflow check, sometimes monthly, some training of
users is required – especially in regards to the alarm system
Airflow is susceptible to air movement
...
Too much
equipment in cabinet
...
g
...
The
inward flow can contaminate samples
...
Two HEPA filters, one for room air and one for contaminated air
Title: Laboratory legislation and safe working practices
Description: This document contains definitions as well as detailed information on the governing bodies control laboratories, risk assessments, hazard grouping, laboratory containment levels, microbiological safety cabinets (MSC) 1 - 3, legislation on genetically modified organisms
Description: This document contains definitions as well as detailed information on the governing bodies control laboratories, risk assessments, hazard grouping, laboratory containment levels, microbiological safety cabinets (MSC) 1 - 3, legislation on genetically modified organisms