Search for notes by fellow students, in your own course and all over the country.
Browse our notes for titles which look like what you need, you can preview any of the notes via a sample of the contents. After you're happy these are the notes you're after simply pop them into your shopping cart.
Document Preview
Extracts from the notes are below, to see the PDF you'll receive please use the links above
1
Aspartate aminotransferase (AST)
AST is a cellular enzyme present in many tissues such as heart, skeletal muscles , kidney, brain, liver,
pancreas or erythrocytes
...
The cytoplasmic
isoenzyme is released into the blood during the moderate cell damage
...
The determination of AST activity in serum is used mainly to assess the liver damage
...
)
gallbladder and biliary tract diseases
(cholangitis, biliary colic)
myocardial diseases
(acute myocardial infarction, heart surgery, resuscitation)
skeletal muscle diseases
(early stage of muscular dystrophy, muscle contusion, prolonged physical exercise)
Reye's syndrome, application of morphine etc
...
It is primarily localized in hepatocytes
...
The determination of ALT activity in serum is used mainly to assess the liver
damage
...
)
gallbladder and biliary tract diseases
(cholangitis, biliary colic)
myocardial diseases
(heart failure with congestion of blood in the liver)
Reye's syndrome
therapeutic application of bovine or porcine heparin etc
...
AST catalyses the transfer of an amino group from L-aspartate to 2-oxoglutarate to form
oxaloacetate and L-glutamate
...
ALT catalyses the transfer of an amino group from L-alanine to 2-oxoglutarate to form pyruvate
and L-glutamate
...
The pyruvate concentration is determined spectrophotometrically in the form of hydrazone, which is
produced by reaction with 2,4-dinitrophenylhydrazine in an alkaline medium
...
MATERIALS AND INSTRUMENTS
commercial diagnostic kits BIO-LA-TEST ALT, AST (Erba Lachema s
...
o
...
Pipette the solutions into the labelled test tubes according to the table
...
Read the absorbance of the sample at 510 nm against the blank
...
Pipette the individual solutions in given order into the labelled test tubes according
to the table
...
Read the absorbances of solutions no
...
1
...
Write the measured values into the table
...
Draw the graph of the dependence of absorbance on the catalytic concentrations