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Title: Genetics Outlline
Description: chart of the enzymes used in DNA replication, transcription and translation and their actions. chart of the actions and processes of DNA replication, transcription, and translation.
Description: chart of the enzymes used in DNA replication, transcription and translation and their actions. chart of the actions and processes of DNA replication, transcription, and translation.
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70S complex
• 80S is formed w/tRNA
Elongation
in P site
• 15-20 a
...
per second
• Elongation
• EF-Tu-GTP to loads
• 2-6 a
...
per second
charged tRNAs to A site • Same steps as proks however:
• Recharging steps
• eEF-1𝛼 replaces EF-Tu
• When tRNA
• eEF-1ß𝛾 replaces EF-Ts
anticodon binds
• eEF2 replaces EF-G
to mRNA, GTP
• Termination:
hydrolysis
• eRF1 recognizes all stop codons
releases EF-Tu(both RF1 & RF2’s job)
GDP so that GTP• eRF3 acts as GTPases, binds &
can be
hydrolyzes GTP, releasing
regenerated
everything
• Performed w/EF• No RRF in Eukaryotes
Tu EF-Ts
complex
• Peptide bond forms
• Bond between amino
acid and tRNA in P is
cleaved
• Peptidyl Transferase
(in 50S) forms Peptide
Met
fMet
•
•
bond between free
amino acid and amino
acid attached to tRNA
in A position
• 23S rRNA has
transferase
activity
• Translocation along mRNA
• EF-G-GTP binds to
the ribosome
• GTP is hydrolyzed
• Uncharged tRNA
moves to the E site,
released
• A site is now empty
for new tRNA
• EF-G is then released
and reused
Termination
• RF1 or RF2 recognize stop
codons, bind @ A site
• Peptidyl transferase cleaves
peptide chain, it’s released
• RF3-GDP binds to
ribosome, releasing other
RF
• GTP replaces GDP and is
hydrolyzed
• Ribosome Recycling Factor
(RRF) binds to A site
• EF-G-GDP attaches to A
site, translocation pushes
uncharged tRNA to E site
• Everything released
(RF3, RRF, ribosomes,
mRNA, EF-G)
Title: Genetics Outlline
Description: chart of the enzymes used in DNA replication, transcription and translation and their actions. chart of the actions and processes of DNA replication, transcription, and translation.
Description: chart of the enzymes used in DNA replication, transcription and translation and their actions. chart of the actions and processes of DNA replication, transcription, and translation.