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Title: Overview of DNA Recombination
Description: BIOCHEMISTRY (2nd YEAR) This is an outline of the section indicating exactly what is covered. Introduction to cloning, construction of recombinant DNA molecules, cloning vectors and indentification of a specific gene These documents are also uploaded separately and will be uploaded together
Description: BIOCHEMISTRY (2nd YEAR) This is an outline of the section indicating exactly what is covered. Introduction to cloning, construction of recombinant DNA molecules, cloning vectors and indentification of a specific gene These documents are also uploaded separately and will be uploaded together
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RECOMBINATION
SECTION OVERVIEW:
I
...
Gene Cloning
B
...
Construction of recombinant DNA molecules
A
...
Preparation of total cell DNA
a
...
Removal of contaminants
ii
...
Plasmid Purification
B
...
Cleavage of the Vector
2
...
Restriction Enzymes
1
...
Cohesive (sticky) ends
3
...
The importance of the site of cleavage
1
...
Restriction of Lambda Phage DNA
a
...
Electrophoresis
ii
...
Estimation of DNA fragment sizes
E
...
Ligation
2
...
Transformation
1
...
Inefficiency
3
...
Function of Cloning
1
...
Purification of recombinant DNA
III
...
Plasmid Vectors
1
...
pBR322
i
...
PUC plasmids
i
...
iii
...
identification of recombinant pUC
why truncated lacZ is used
Identifying a specific gene
A
...
Process
B
...
Gene Library
2
...
Detection of cloned gene
b
...
Gene of interest is Unavailable
1
...
Gene is a member of a family of genes that are related
Title: Overview of DNA Recombination
Description: BIOCHEMISTRY (2nd YEAR) This is an outline of the section indicating exactly what is covered. Introduction to cloning, construction of recombinant DNA molecules, cloning vectors and indentification of a specific gene These documents are also uploaded separately and will be uploaded together
Description: BIOCHEMISTRY (2nd YEAR) This is an outline of the section indicating exactly what is covered. Introduction to cloning, construction of recombinant DNA molecules, cloning vectors and indentification of a specific gene These documents are also uploaded separately and will be uploaded together