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Unit 3 - Assignment 1 - P2
Microbiology - Testing bacterial response to disinfectants
Hypothesis
The more concentrated the disinfectant is, the more bacteria it will kill
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Equipment
•
•
•
•
•
•
•

1ml pipettes x10
Test tubes x5
50ml of sterile water
Dettol
Petri dishes x6
Nichrome loop
Water bath at approximately 80oC

•

Culture of Staphylococcus Aureus

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9ml of sterile agar in bottles x5

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10ml of sterile agar in bottles

Method
1
...
0
1
...
0ml of 1 in 10 Dettol
1
...
0ml of 1 in 1,000 Dettol

0
...
0
9
...
0
9
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Keeping the bottles of agar in the water bath (80oC); to each bottle of agar add 1ml of the
appropriate Dettol concentration
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0
9
...
0
9
...
0

Appropriate Dettol
concentration (ml)
0
...
0 of 1 in 10,000
1
...
0 of 1 in 100
1
...
0

1
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Mix Dettol and agar well and pour into relevant petri dish
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Allow the agar (and Dettol) to set well
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Research
Explains about
how different
disinfectants and
bacteria and the
effects of them
...
e
...

Shows full report
on same
microbiology/disi
nfectant
investigation
...

Explains about
how different
disinfectants and
bacteria and the
effects of them
...
e
...

Shows detailed
procedures
needed to carry
out experiment
and a step by step

Reference
ALEKSANDRA, Z
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(2013) Chemik
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chemikinternational
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p
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[Accessed: 12th November 2014]
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(2002-2003) The Effect of Various Disinfectants on Bacteria
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selah
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wa
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html
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Society for General Microbiology (SGM)
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[Online] A Manual
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Available from:
https://onedrive
...
com/view
...
[Accessed: 12th November 2014]
...


Unit 3 - Assignment 1 - M1
Investigation research
(microbiology)
Disinfection includes
techniques of controlling
microbes by the use of
chemicals to kill them
...
Disinfectants have a
purpose to interrupt the
transport of microbes between
the source of infection and
healthy individuals
...

In order for people to stay
healthy, they must use a
disinfectant that will kill
microbes effectively, with no
damage to people themselves,
i
...
corrosion of skin, irritation,
rashes, inflammation, etc
...
Bacteria
reproduce by a process called
fission, this is when a cell
divides in to two parts,
bacteria does this repeatedly
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& MARTA, S
...
[Online] Available
from: http://www
...
com/year-2013/year2013-issue-2/comparison-of-the-effectiveness-of-antibacterialeffect-of-disinfectants-on-microorganisms-isolated-from-theepidermis-ziembinska-a-szpindor-m/
...
67
...


CHARLEY, W
...
[Online] Available from:
http://www
...
k12
...
us/soar/sciproj2003/charleyw
...

[Accessed: 12th November 2014]
...

There are four types of
bacteria and they differ
because of the shape:

There are some types of
bacteria that can live with or
without oxygen
...
Also bacteria can
differ due to it being harmful
or harmless
...
Harmful bacteria can
be cause diseases like cholera,
TB, typhoid, the whooping
cough, etc
...
They can
also get in by little cuts on the
surface of ones skin or by the
nostrils or mouth
...
They
are vey efficient when used to
clean hospital units; the
hospital disinfectants are far

more effective than the normal
house hold ones
...

• Straight wire
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• Forceps
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• Teat
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• Universal bottle
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• Petri dishes
...

• Protective clothing:
goggles, lab coat, etc
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• Autoclave
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• Disinfectants
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• Incubator
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• Thermometer
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• Microscope
...

• Alcohol - Must be kept
under adult
supervision as student
may consume some
...
Also must be
kept away from certain
members of staff, as
working in a college
environment can be
stressful
...

• Steriliser
...

Risk assessment
Risks include:
Spillages - Must report
immediately to a member of
staff
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Society for General Microbiology (SGM)
...
[Online] A Manual
...
Available from:
https://onedrive
...
com/view
...
[Accessed: 12th
November 2014]
...
g
...

Broken glassware - Must not
touch broken glass with
unprotected hands as it could
result in an injury
...

Splashing on clothing and
contact with skin - This could
cause irritation, corrosion,
inflammation, rashes or burns
...
Clothing should be
washed with disinfectant
...

Sterilisation - e
...
alcohol
...

Storage - cupboards
...

Techniques, e
...
using a wire
loop or using a pipette
...


How reliable these sources are to my investigation and why?
Society for General Microbiology (SGM)
...
SGM also produces and distributes a range of resources to colleges and schools to teach
microbiology
...
This includes the sterilisation of the equipment and materials such
as the wire loop, glassware, metal forceps, etc
...
The e-book also explains the preparation needed before carrying out any
procedures; this includes a risk assessment and safety guidelines
...

This source is relevant for my investigation as it shows exactly what procedures I should carry out
and what equipment I need to do it
...


The Effect of Various Disinfectants on Bacteria
This website is a reliable source as it is detailed, the author displays a range of sources and
references to back up her information whilst including a bibliography and acknowledgement to the
people who helped her
...

This website gave me the information and a summary of what my investigation would be like
...
She also shows the equipment and substances she used including the
quantities and measurements of them
...
She gives a short explanation of the results and what
happened including a detailed and labelled table and two bar charts
...
Finally, this source
explains the characteristics of different bacteria and the effect on different disinfectants on bacteria
and the history
...
Also, the behaviour of different bacteria
and disinfectants
...
It says that
the research is taken place at in the Environmental Biotechnology Department, in the Silesian
University of Technology, Poland
...
For
example, hydrogen peroxide is used to disinfect wounds
...
In addition, it explains some of
the history of disinfectants and how different concentrations can act when added to bacteria
...
Finally they summarised it all up in a
conclusion explaining what they did, their results and what and what factors would have made a
difference to the results
...




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