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CHAPTER 2 : Basic components of living systems
Magnification and calibration
Magnification, resolution and the magnification formula
-Magnification is how many times larger the image is than the actual size of the
object being viewed
-Interchangeable objective lense adjust magnification (compound light microscope)
-Resolution determines amount of detail that can be seen
-High resolution = more visible details
-Resolution is limited by diffraction of light as it passes through light and samples
-Diffraction = tendency of light waves to spread as they pass close to physical
structures
-In optical microscopy structures that are closer than half the wavelength of light
cannot be seen separately (resolved)
-Increase resolution by beams of electrons which have a wavelength of a thousand
times shorter than light
-Electron beams are still diffracted
-Shorter wavelength means individual beams can be much closer before they
overlap
-Objects that are small and close together can be seen separately without diffraction
blurring the image
Calculation for magnification
-Magnification = image / actual size



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