Search for notes by fellow students, in your own course and all over the country.
Browse our notes for titles which look like what you need, you can preview any of the notes via a sample of the contents. After you're happy these are the notes you're after simply pop them into your shopping cart.
Title: GENETIC TECHNOLOGY
Description: Notes include principles of genetic engineering and processes involved with diagrams
Description: Notes include principles of genetic engineering and processes involved with diagrams
Document Preview
Extracts from the notes are below, to see the PDF you'll receive please use the links above
What is Recombinant DNA (rDNA)?
The altered DNA, with the introduced
nucleotides from two or more sources
...
Selective breeding involves whole sets of genes
being transferred while in genetic breeding,
often results in a transfer of a single gene
...
There is the removing a gene (or genes) with
the desired characteristic from one organism
and transferring the gene (using a vector)
into another organism where the desired
gene is then expressed
Enzymes Required for GE (4)
Restriction endonucleases (enzymes) – cuts
the DNA strands so that the desired gene can
be isolated or spliced (inserted) into a vector
Reverse transcriptase – reverses
transcription to produce a single-strand
complementary DNA (cDNA) from an mRNA
strand with the code for the desired gene
DNA polymerase – used to convert the singlestranded cDNA into a double-stranded DNA
molecule of the desired gene
DNA ligase – is used to splice (insert) the gene
into the vector
Vectors
Vectors are used to transfer the desired
genes into a foreign cell
Plasmids are used to transfer the desired
genes
POLYMERASE CHAIN REACTION
Polymerase chain reaction (PCR)
a common molecular biology technique used in
most applications of gene technology
Used for rapid production of very large number
of copies of a particular fragment of DNA
...
The ‘bulk up’ of DNA
...
PCR Requirements
Target DNA or RNA being amplified
Primers (forward and reverse) – these are
short sequences of single-stranded DNA
that have base sequences complementary
to the 3’ end of the DNA or RNA being
copied
...
Free nucleotides – used in the
construction of the DNA or RNA strands
Buffer solution – to provide the optimum
pH for the reactions to occur in
...
GEL ELECTOLOSIS
Promoter
The promoter (an example of a length of noncoding DNA that has a specific function) is the
region of DNA that determines which gene
will be expressed
...
Gene Marker
A marker is a gene that is transferred with the
desired gene to enable scientists to identify
which cells have been successfully altered
and now contain recombinant DNA
Title: GENETIC TECHNOLOGY
Description: Notes include principles of genetic engineering and processes involved with diagrams
Description: Notes include principles of genetic engineering and processes involved with diagrams