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Title: Determining metal ion concentration by Beer,s Law.pdf
Description: Eperimnet for Determining metal ion concentration by Beer,s Law.pdf
Description: Eperimnet for Determining metal ion concentration by Beer,s Law.pdf
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Introduction:
The motivation behind this lab was to figure out how to utilize a spectrophotometer to gauge the
convergence of a metal particle
...
A spectrophotometer is a
valuable instrument since it decides absorbance by estimating the decline in light as it goes
through an example
...
These qualities are then, at that point,
changed over to the Absorbance (A) by taking the log of the proportion of those intensities,
A ≡ log I o
...
Also, the idea of the matter impacts the
measure of light assimilated
...
This worth is likewise explicit for that frequency
...
Procedure:
The lab strategies comprised of three sections
...
To set up the standard arrangements, a burette containing 1
...
The five standard arrangements from the table given in lab were ready
...
When the frequency was set, the
spectrophotometer was focused utilizing 0
...
The absorbance
of every one of the standard arrangements and the obscure were estimated
...
The slop and still up in the air and showed on the chart
...
Results/Data:
Data for calibration curve using standard solutions
Sample #
Volume of
Cu(NO3)2 stock
solution, mL
Total
Volume,
mL
blank
0
25
1
1
25
2
5
3
10
25
25
4
15
25
5
20
25
Solution Concentration, mol/L
Absorbance
M1V1=M2V2
M2= (1
...
00 mol/L
M1V1=M2V2
M2= (1
...
04 mol/L
0
...
0M*5mL)/(25mL)
M2= 0
...
0M*10mL)/(25mL)
M2= 0
...
0M*15mL)/(25mL)
M2= 0
...
0M*20mL)/(25mL)
M2= 0
...
456
0
...
678
0
...
850
For unknown sample of metal
ABSORBANCE SAMPLE #1: 0
...
567
Equation of line
y= 1
...
2365x
Absorbance= (1
...
350/1
...
283 mol/L
SAMPLE #2: Concentration of Cu(NO3)2 in measured
sample (from calibration curve), mol/L
Concentration= (0
...
2365)
Concentration= 0
...
6
Absorbance
0
...
4
0
...
2
Calibrators
0
...
1
0
...
3
0
...
5
0
...
An alignment
bend was made to get a precise worth of ε ∙ ℓ
...
2365
...
2365x, where y is the absorbance worth
and x is the arrangement focus
...
2365
...
350 by
1
...
This brought about a molar convergence of 0
...
The
second example molar fixation was determined by partitioning 0
...
2365
...
459 mol/L for test #2
...
Title: Determining metal ion concentration by Beer,s Law.pdf
Description: Eperimnet for Determining metal ion concentration by Beer,s Law.pdf
Description: Eperimnet for Determining metal ion concentration by Beer,s Law.pdf