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Bacterial culture and Identification – Basics
All bacteria can be cultured and identified by using various tests and using certain growth medium
Bacteria can be cultured in two ways: a petri dish using agar gel or in suspension using a liquid
media
...
e
...
This is using liquid
medium and can quantify bacterial growth
through spectrophotometry
...

1
...
Finally after incubation after 46-72
hours at 37°C – Bacterial colonies can
form
...
Four inoculation sites are made
by rubbing the loop/swab
sideways through the pathway
shown in the diagram on the right
...

Cotton buds (blue) can
be used as long as it’s
sterile when used
...


Rod shaped bacteria are long and boxed shaped
...

Escherichia coli 0157

(How many different rods can you really get?)

Spirium bacteria are visually different from the
previous two, its long and thin
...
They are halotolerant (ie can live in a high salt environment)
...
Other
bacteria’s growth is inhibited by the salt content
...

Charcoal yeast agar – used to identify Legionella pneumophilia (causes legionnaires disease)

*Please see other notes for Gram Stain resources



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