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Title: Hematoxylin and Eosin Stain procedure
Description: The procedure of staining a histological sample with Hematoxylin and Eosin stain.
Description: The procedure of staining a histological sample with Hematoxylin and Eosin stain.
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Haematoxylin and Eosin Stain Protocol
Haematoxylin and Eosin stain is the most common stain used in histopathology; this is because it
gives a clear distinction between the nucleus and cytoplasm
...
Firstly the sample is hydrated into decreasing concentrations of alcohol until left in water; this is
called taking the sample to water
100%
95%
95%
70%
70%
Water
1- Place Haematoxylin on the sample for 10 minutes
2- Rinse in distilled water
3- Place acid alcohol on the sample for 5-10 seconds – no more than that as the stain will be
too faded
4- Rinse in distilled water
5- Place in Scott’s tap water (slightly alkali water) for 10 minutes – to blue the sample
6- Rinse in distilled water
7- Place Eosin on the sample for 10 minutes
8- Rinse in distilled water
9- Place in xylene
10- Dehydrate the sample in increasing concentrations of alcohol (opposite to above image)
11- Use DPX to stick over slip on sample and leave to dry
Title: Hematoxylin and Eosin Stain procedure
Description: The procedure of staining a histological sample with Hematoxylin and Eosin stain.
Description: The procedure of staining a histological sample with Hematoxylin and Eosin stain.