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Title: Phlebotomy Notes
Description: Everything you need to know!! Detailed notes, Order of Draw, Tube Additives Procedures: Bleed Test- Glucose Test- Capillary Test, Preparing Blood Smear, Venipuncture, vocabulary, Definitions,
Description: Everything you need to know!! Detailed notes, Order of Draw, Tube Additives Procedures: Bleed Test- Glucose Test- Capillary Test, Preparing Blood Smear, Venipuncture, vocabulary, Definitions,
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Blood Specimens in Phlebotomy
• Whole Blood- (For the most part) Not Centrifuged
•
Serum- Centrifuged
•
Plasma- Centrifuged
For Serum specimen:
1
...
Tube Additives
1
...
Initiating (starting or activating) the process of coagulation
3
...
Anticoagulant- Prevent blood from clotting/ Let the blood in
collection tube stay in liquid form
2
...
Silica=absorbs moisture/ Desiccant)
3
...
A
...
B
...
Light Blue - Fully Fill Tube- (9 to 1 ratio/ 9 parts blood to 1
part Sodium citrate)
(3-4 inversions)
Additive: Sodium citrate (anticoagulant)
Specimen: Plasma
Laboratory Uses:Coagulation studies: PT, PTT, and
Fibrinogen
(PT- ProThrombin time)
(PTT- Partial Thromboplastin time)
3
...
Plastic / B
...
)
Additive: Plastic: Clot activators - Glass: No Additive
Specimen: Serum
Laboratory Uses: Chemistry, Serology, Immunology, &
Blood Bank
Plastic: (5 inversions)
Glass: No additive - no inverting
Serology= Study of serum
3
...
Gold / D
...
A
...
B
...
Lavender (8-10 inversions)
Additive: EDTA K2 (Potassium EDTA), EDTA K3
Specimen: Whole Blood
Laboratory Uses: Hematology Studies: Complete Blood
Count(CBC), WBC count, Hemoglobin(iron protein), Hematocrit(%
of Blood), Platelet count, Erythrocytes sedimentation Rate (ESR),
RBC, Reticulocyte, and differential count
Reticulocyte: immature RBC first 24hrs
6
...
Royal Blue (8-10 inversions)
Additive: Sodium or Sodium EDTA or No Additive
Specimen: Plasma or Serum
Laboratory Uses: Chemistry trace elements: Toxicology
trace metals, lead, and nutrition analysis
2
...
Orange (5-6 inversions)
Additive: Thrombin (Clot activator) or T
appropriate gauge)
•
Reapply tourniquet
•
With fingers of non-needle carrying hand pull skin taut
•
Dispose of gloves and supplies into containers
•
Remove gloves
•
Package specimen for transport
•
Apply bandaid
•
Check the patient for hemostasis by inspecting the site of
incision
•
Blood collection tubes labeling must be done to facility
standards
•
•
With needle carrying hand insert needle at the appropriate
angle
•
Fill tubes in correct order of draw
•
Remove tourniquet- 60sec
...
•
Fill syringe but pull plunger back slowly
•
insert needle at appropriate angel
•
Pull patient skin taut to anchor skin
•
Reapply tourniquet
•
Connect syringe to needle, inspect needle
•
Disinfect site with alcohol
•
Package specimen for transport
•
Remove gloves
•
Dispose of gloves
•
Wash hands
•
Title: Phlebotomy Notes
Description: Everything you need to know!! Detailed notes, Order of Draw, Tube Additives Procedures: Bleed Test- Glucose Test- Capillary Test, Preparing Blood Smear, Venipuncture, vocabulary, Definitions,
Description: Everything you need to know!! Detailed notes, Order of Draw, Tube Additives Procedures: Bleed Test- Glucose Test- Capillary Test, Preparing Blood Smear, Venipuncture, vocabulary, Definitions,