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Title: Phlebotomy Notes
Description: Everything you need to know!! Detailed notes, Order of Draw, Tube Additives Procedures: Bleed Test- Glucose Test- Capillary Test, Preparing Blood Smear, Venipuncture, vocabulary, Definitions,
Description: Everything you need to know!! Detailed notes, Order of Draw, Tube Additives Procedures: Bleed Test- Glucose Test- Capillary Test, Preparing Blood Smear, Venipuncture, vocabulary, Definitions,
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Blood Specimens in Phlebotomy
• Whole Blood- (For the most part) Not Centrifuged
•
Serum- Centrifuged
•
Plasma- Centrifuged
For Serum specimen:
1
...
Tube Additives
1
...
Initiating (starting or activating) the process of coagulation
3
...
Anticoagulant- Prevent blood from clotting/ Let the blood in
collection tube stay in liquid form
2
...
Silica=absorbs moisture/ Desiccant)
3
...
A
...
B
...
Light Blue - Fully Fill Tube- (9 to 1 ratio/ 9 parts blood to 1
part Sodium citrate)
(3-4 inversions)
Additive: Sodium citrate (anticoagulant)
Specimen: Plasma
Laboratory Uses:Coagulation studies: PT, PTT, and
Fibrinogen
(PT- ProThrombin time)
(PTT- Partial Thromboplastin time)
3
...
Plastic / B
...
)
Additive: Plastic: Clot activators - Glass: No Additive
Specimen: Serum
Laboratory Uses: Chemistry, Serology, Immunology, &
Blood Bank
Plastic: (5 inversions)
Glass: No additive - no inverting
Serology= Study of serum
3
...
Gold / D
...
A
...
B
...
Lavender (8-10 inversions)
Additive: EDTA K2 (Potassium EDTA), EDTA K3
Specimen: Whole Blood
Laboratory Uses: Hematology Studies: Complete Blood
Count(CBC), WBC count, Hemoglobin(iron protein), Hematocrit(%
of Blood), Platelet count, Erythrocytes sedimentation Rate (ESR),
RBC, Reticulocyte, and differential count
Reticulocyte: immature RBC first 24hrs
6
...
Royal Blue (8-10 inversions)
Additive: Sodium or Sodium EDTA or No Additive
Specimen: Plasma or Serum
Laboratory Uses: Chemistry trace elements: Toxicology
trace metals, lead, and nutrition analysis
2
...
Orange (5-6 inversions)
Additive: Thrombin (Clot activator) or Thrombin w/
Gel
Specimen: Serum
Laboratory Uses: STAT Serum Chemistries
4
...
Blood culture tube- Yellow SPS or (sterile)
2
...
Serum- Tube w/o clot activator (Red:Glass) & w/ clot
activator (Red:Plastic), Serum Separator Tube (SST)
4
...
EDTA- Tube w/o gel separator (Lavender, Pink,) & EDTA
tube w/ Plasma Preparation Tubes (PPTs) (White)
6
...
-1min
...
Lanet- Width-2
...
0mm
Middle finger or Ring finger
Dermal puncture order of draw
Lets get Lit, Guys you ready?
Lavender- EDTA
Green- lithium heparin
Light Green- lithium heparin w/gel
Gray- NaF/Na2EDTA
Yellow- Clot activator
Red- No Additive
Centrifuge machine
Spins and force to separate heavier and lighter particles
Lighter particles are towards the top\ heavior moves to the
bottom
Must be balanced on opposite side
RPM- rotation per minute
Phlebotomy procedures 1
Procedure
1
...
Identify the speed and duration
3
...
Secure rotor lid properly, close centrifuge lid
5
...
When cycle is over rotor has completely stopped spinning
Specimen processing
1
...
Directly centrifuge
Position patient comfortably
•
Check if patient is on medication or dietary restriction
•
Prep- Prepare & disinfect incision site
•
Apply blood pressure cuff
•
Explaining procedure to patient
•
Washing hands & Donning PPE
•
Assemble equipment & supplies
•
ID patient- Full name/ DOB/
•
ID yourself & procedure
•
Greet patient
•
Bleeding time test procedure
• Verify physician's order
•
Position device on palmar surface on forearm
•
Blot Blood in 30sec
Title: Phlebotomy Notes
Description: Everything you need to know!! Detailed notes, Order of Draw, Tube Additives Procedures: Bleed Test- Glucose Test- Capillary Test, Preparing Blood Smear, Venipuncture, vocabulary, Definitions,
Description: Everything you need to know!! Detailed notes, Order of Draw, Tube Additives Procedures: Bleed Test- Glucose Test- Capillary Test, Preparing Blood Smear, Venipuncture, vocabulary, Definitions,