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Title: Isolation of Chloroplast
Description: It includes the principle and procedure to isolate chloroplast to be used during performing practical.
Description: It includes the principle and procedure to isolate chloroplast to be used during performing practical.
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EXPERIMENT No:
ISOLATION OF CHLOROPLAST
INTRODUCTION
Isolated chloroplasts are required to study the electron transport system of photosynthetic apparatus
...
Any one of
these methods can be followed, provided the isolated chloroplasts are biochemically active as the
chloroplasts in vivo
...
PRINCIPLE
The cell organelles depending upon their size and weight sediment at different centrifugal fields
...
REAGENTS
Isolation Medium: Weigh 2
...
8g Sorbitol (0
...
168g NaCl (20mM);
0
...
6H2O (3mM) and dissolve in one liter of distilled water
...
8
...
8M (52
...
5M (43
...
2M
(35
...
6% (19
...
2), 0
...
Cut spinach leaf into small bits
...
2
...
3
...
4
...
5
...
6
...
7
...
5 to 1
...
68
8
...
8M, 6ml
1
...
2M and 3ml 0
...
9
...
10
...
69
Title: Isolation of Chloroplast
Description: It includes the principle and procedure to isolate chloroplast to be used during performing practical.
Description: It includes the principle and procedure to isolate chloroplast to be used during performing practical.