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Title: Effect of Non-canonical Structure on UV Absorption
Description: Anatomy and physiology course
Description: Anatomy and physiology course
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Spectroscopic Properties of Noncanonical Nucleic Acids
4
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1
Effect of Non-canonical Structure on UV Absorption
One of the most important properties of nucleic acids is ultraviolet (UV) absorption
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The absorption maximum is near 260 nm with extinction coefficients about
10 000 M−1 cm−1, which is derived from π–π* transitions of the nucleobase [1]
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at 295 nm
275 nm
R
1
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8
0
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4
0
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1 UV spectroscopic property of nucleic acids structure
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The red arrows indicate the induced
dipole moments triggered by neighboring base (left) or counter part of the Watson-Crick
base (right)
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Source: Crothers et al
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(c) Absorbance change of G4 structures
observed UV melting measurement
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Because the neighboring dipole moment induces the
transition dipole moment (Figure 4
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In the case of
canonical duplex structures, the base stacking decreases the transition dipole
moment of bases, which makes UV absorbance at 260 nm of duplex smaller than
that of single-stranded state
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Using
the changes in absorbance by the formation of the structure, the thermal stability of
duplexes is determined by UV melting analysis (see Chapter 3)
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As this spectroscopic effect
depends on the chemical structure and geometry of nucleobase, the property of
extinction coefficients of the non-canonical structure differs from each other
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The base pair
of G4 consisted of four bases of guanine base via Hoogsteen hydrogen bonding
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1b)
[1]
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Another tetraplex
structure of i-motif, which contains mutually orthogonal hemiprotonated cytosine
base pairs
...
This hyperchromicity of G4 and i-motif provides useful
information in the UV melting analysis as well as the hypochromicity of duplexes
(see Chapter 3)
...
Thus, tracking the temperature
dependency of UV absorbance at 295 nm enables to observe the thermal folding
and unfolding of G4 and i-motif structures (Figure 4
...
In the case of the triplex
structure, the strand paired with Hoogsteen hydrogen bonding showed
hypochromicity as well as the canonical Watson–Crick base pairs
Title: Effect of Non-canonical Structure on UV Absorption
Description: Anatomy and physiology course
Description: Anatomy and physiology course