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Title: General MIcrobiology LAB Record ( Experiment 1)
Description: pure culture, in microbiology, a laboratory culture containing a single species of organism. A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into a new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample manyfold before inoculating the new medium. Both methods separate the individual cells so that, when they multiply, each will form a discrete colony, which may then be used to inoculate more medium, with the assurance that only one type of organism will be present. Isolation of a pure culture may be enhanced by providing a mixed inoculum with a medium favoring the growth of one organism to the exclusion of others.
Description: pure culture, in microbiology, a laboratory culture containing a single species of organism. A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into a new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample manyfold before inoculating the new medium. Both methods separate the individual cells so that, when they multiply, each will form a discrete colony, which may then be used to inoculate more medium, with the assurance that only one type of organism will be present. Isolation of a pure culture may be enhanced by providing a mixed inoculum with a medium favoring the growth of one organism to the exclusion of others.
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REGULATIONS INSIDE THE LABORATORY
Enter the laboratory with a clean coat to protect your clothes
...
Keep the desk free non-essential materials
...
Many microscopic organisms any be pathogenic, therefore avoid any hand to
mouth operations
...
Whenever a living culture is spilled on the desk or floor at once disinfect the
place
...
Handle all cultures
carefully according to the instructions
...
Before starting an experiment you should know you are going to do and take note
of all the observations
...
Do not have valuables in the lab coat
...
Speak quietly and avoid unnecessary movements around lab to prevent
distractions that cause accidents
...
DATE: 16/08/2022
EXPERIMENT NO: 1
PURE CULTURE TECHNIQUES
A microbiology culture or microbial culture is a method of multiplying organisms by
letting them reproduce in pre-determined culture media under controlled laboratory
conditions
...
A pure culture may originate from cell or single cell or
single organism in which case cells are genetic clones of one another
...
In this by means of a
transfer loop a portion of bacterial specimen was placed on the surface of agar medium
and specimen was streaked over the surface in the petri-dish with a platinum wire loop
...
In some areas of the plate, to ensure that colony
developing from one cell was not merged with that of growing from another
...
Next day isolated colonies can be observed under the
nutrient agar plate
...
A pure culture is usually derived from
mixed culture containing many species by method that separate the individual cell so
that when they multiply each will form a individual distant colony, which may then be
used to establish new culture with the assurance that only one type of the organism will
be present
...
SERIAL DILUTION:
A serial dilution is the stepwise dilution of a substance in solution usually the dilution
factor at each step is constant resulting in a geometric progression of the concentration
in a logarithmic fashion
...
1M, 0
...
serial dilution, are
used to accurately create highly diluted solution
...
STREAK PLATE:
This method is a rapid qualitative isolation method
...
SPREAD-PLATE:
This technique requires that a pre-dilution mixture of microorganisms be used
...
POUR-PLATE:
This technique requires a serial dilution of the mixed culture by means of a loop pipette
...
Title: General MIcrobiology LAB Record ( Experiment 1)
Description: pure culture, in microbiology, a laboratory culture containing a single species of organism. A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into a new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample manyfold before inoculating the new medium. Both methods separate the individual cells so that, when they multiply, each will form a discrete colony, which may then be used to inoculate more medium, with the assurance that only one type of organism will be present. Isolation of a pure culture may be enhanced by providing a mixed inoculum with a medium favoring the growth of one organism to the exclusion of others.
Description: pure culture, in microbiology, a laboratory culture containing a single species of organism. A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into a new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample manyfold before inoculating the new medium. Both methods separate the individual cells so that, when they multiply, each will form a discrete colony, which may then be used to inoculate more medium, with the assurance that only one type of organism will be present. Isolation of a pure culture may be enhanced by providing a mixed inoculum with a medium favoring the growth of one organism to the exclusion of others.