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Title: PPBM: 1.10.2 Introductory of microbiology
Description: This pdf about prepration of different types of culture media with is useful for growth of fungi and bacteria
Description: This pdf about prepration of different types of culture media with is useful for growth of fungi and bacteria
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Media
Culture
Culture media
• A culture medium is a solid or liquid preparation
of nutrient or combination of nutrients used to
grow, transport, and store microorganisms
...
• Culture media is useful in the identification and
characterization of microorganisms
Medium (Media)
The continued growth and survival of microorganisms depend
on an adequate supply of nutrients and a favourable growth
environment
...
Media are liquid, semisolid, or solid
...
...
• On purpose/functional use/ application(1) Basal media
(2) Enriched media
(3) Selective media (4) Indicator media
(5) Transport media (6) Storage media
...
• Prepared artificially by adding
organic and inorganic chemicals
• These media are useful for
nutritional
and
metabolic
studies
...
Non-synthetic Media (or Complex Media) Natural media
• A medium in which the exact chemical composition of
one or each of the constituents is not known is
referred to as non-synthetic medium, undefined
medium, or complex medium
...
A completely solid medium
requires an agar concentration
of about 1
...
0%
...
Nutrient-agar medium, Nutrientgelatin medium, Potato DextroseAgar medium
• Agar is a complex polysaccharide
(carbohydrate) mainly D- galactose
• Produced from various red algae
belonging to genera Gelidium,
Gracilaria,
Gigartina
and
Pterocladia
...
(c) Used for isolation of bacteria as pure culture
...
e
...
Ex
...
Advantages
• To study the production of enzymes
and toxins
• To study soluble products of
bacteria
• To study the fermentation studies
Semi-solid Media
• Semi-solid media contain a
smaller amount (1% or less)
of agar which imparts a
“custard consistency”
...
: Cystine trypticase-agar
medium
Advantages
• Useful for the cultivation
of micro-aerophilic bacteria
• For
determination
of
bacterial motility
Based on purpose
BASAL MEDIA
...
• Basal media are basically simple media that
supports most non-fastidious bacteria
...
: Nutrient broth, nutrient agar and peptone water
...
• It contain antibiotics, dyes or specific chemicals
that inhibit the growth of other microbes
• Ex
...
a color or pH
change)
...
Ex
...
Enriched Media
•
•
•
•
•
Enrichment culture is that in which the
growth of a particular microorganism is
favoured as against a mixed population
by adjusting the nutritional requirements
and environmental factors
...
Enrichment culture is typically used
as broth medium
Enrichment medium is used to increase
the relative concentration of certain
microorganisms
...
Blood agar for isolation of
Streptococcus, chocolate agar for
isolation of Neisseria and Haemophilus
• Selective and enrichment media are designed
to
inhibit
unwanted
microbes
or
contaminating bacteria and help to recover
pathogen from a mixture of bacteria
...
• This type of medium mainly
used for Temporary storage of
specimens being transported to
the laboratory for cultivation
Title: PPBM: 1.10.2 Introductory of microbiology
Description: This pdf about prepration of different types of culture media with is useful for growth of fungi and bacteria
Description: This pdf about prepration of different types of culture media with is useful for growth of fungi and bacteria