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Title: IBDP-Internal Assesment-SL-IA
Description: Complete biology IA with data, Picture, Calculation, Row data, process data, conclusion, and Result.
Description: Complete biology IA with data, Picture, Calculation, Row data, process data, conclusion, and Result.
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The Effect of Solute Concentration (Glucose and NaCl) on Osmolarity in
Solanum tuberosum (Russet Potato) Cells
...
Introduction
Ever since I was a little kid, I loved going to my grandma's house for her famous mashed potatos
dish
...
She said it would make them mushy or tough, depending on the type of
solution
...
Later in science class, I learned about osmosis and how water moves in and out of cells
...
I got inspired to
test this idea out
...
I wanted to see if I could take this basic cooking tip I learned growing up and get some
hard data on how osmolarity impacts potato cell osmosis
...
Even the simplest kitchen
skills can connect back to the world of science in cool ways
...
Research Question
"How do the osmolarity levels of Solanum tuberosum (Russet Potato) cells change when
exposed to sodium chloride (NaCl) and glucose solutions at concentrations of 0
...
1M,
0
...
3M, 0
...
5M, respectively?"
3
...
This movement of solvent molecules through selective membrane channels
seeks to solute concentrations on both sides
...
On one side is pure water, creating
a hypotonic solution, while on the other is a concentrated
chocolate solution, forming a hypertonic solution
...
Driven by this
concentration gradient, with lower solute concentration on
Figure 1
...
This net flow
continues until equilibrium is achieved, analogous to how water
enters plant or animal cells
...
The selectivity of cellular membranes provides the basis for osmotic flow
...
The phospholipid tails are hydrophobic and would not permit water flow without these
2
integral proteins
...
Osmosis regulates cellular turgor pressure,
maintains hydration, absorbs nutrients, and eliminates wastes by virtue of these channels
within the semi-permeable membrane (BioLibretext, 2023)
...
But when you look
closer, you realize plant cells have some totally unique structures not found in animal cells
...
One big difference is the plant cell wall, that rigid box
around the cell membrane
...
The primary cell
wall is
5
...
1 g) - for measuring the mass of potato sticks before and
after osmosis
...
Ruler - to measure the length of potato sticks accurately
...
Stopwatch - to time the duration of potato stick immersion accurately
...
Figure
...
1 g, which could lead to slight
variations in the mass measurements of the potato sticks
...
Evaporation from the beakers over the duration of the experiment could slightly alter
solution concentrations
...
Risk and Safety Considerations (ChatGPT, 2024):
β’
β’
β’
Be cautious with kitchen knives when cutting potato samples to avoid injury
...
Keep NaCl and glucose solutions away from children and pets
...
DATA COLLECTION AND PROCESSING (NaCl)
This the data collected from the experiment with NaCl solutions with varying concentrations
as shown in the following picture:
figure
...
0
5
...
0
4
...
0
5
...
9
5
...
8
4
...
8
noticeably
swollen
0
...
0
4
...
0
4
...
0
Firm and
smooth
5
...
7
4
...
8
5
...
2
5
...
0
4
...
0
5
...
4
4
...
6
4
...
6
mildly swollen
0
...
0
4
...
0
4
...
0
Firm and
smooth
5
...
2
4
...
1
5
...
0
Firm and
smooth
4
...
4
0
...
0
5
...
0
4
...
0
4
...
0
4
...
0
5
...
0
4
...
9
5
...
8
3
...
7
4
...
1: Quantitative and qualitative data from NaCl experiment
To understand how the potato sticks were affected by the saltwater solutions, I first measured
how much their weight changed
...
The formula we used was:
πΆβππππ ππ πππ π = πΉππππ πππ π β πΌπππ‘πππ πππ π
...
The formula for this is:
πππππππ‘πππ πΆβππππ ππ πππ π = (
β πππ π
ππππ‘πππ πππ π
) Γ 100%
...
2M NaCl concentration:
1
...
4gβ5
...
4g
...
The percentage change in mass is
...
4
Γ 100 = 8
...
0
8
To calculate the average percentage change in mass and the standard deviation I used Excel
...
The specific numbers I found from these
calculations are shown in the following table
...
0
0
...
0
0
...
9
0
...
0
25
...
0
22
...
0
20
...
56
0
...
6
0
...
9
0
...
8
12
...
5
22
...
0
16
...
6
3
...
2
0
Title: IBDP-Internal Assesment-SL-IA
Description: Complete biology IA with data, Picture, Calculation, Row data, process data, conclusion, and Result.
Description: Complete biology IA with data, Picture, Calculation, Row data, process data, conclusion, and Result.