Notesale: Turn your study into money

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Emma smith

The effect of changing enzyme concentration on rate of reaction
Aim= to see how the enzyme concentration affects the rate of the reaction
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Hypothesis- I think that the more concentrated the enzyme the quicker the rate of a reaction
because there will be more active sites to break down more of the substrate (protein) therefore
making it quicker
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5%, 0
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3%, 0
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1%, 0% of trypsin
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Test tube rack
Measuring cylinder
Marker pen

Method:
1
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Get your beaker of trypsin and measure out 20cm ³ with a measuring cylinder, then
place in all test tubes in the test tube rack
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Then with a marker pen draw and “X” on the opposite side to where you’re looking,
make sure the x is hidden by the solution when you look through the test tube
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Before you add the enzyme get your stop clocks ready in line with each test tube, and
label each test tube, furthermore with the thermometer check all the solutions are the
same temperature
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Pour each concentration into each test tube making sure they are all the same volume
and start the stop clock
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When you can see the “X” on the other side of the tube, stop the clock and record your
results
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Wash splashes from the
skin as quickly as possible
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Ensure students inform you if any trypsin gets in
their eyes
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Concentration of enzyme %
0
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4
0
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2
0
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08
8
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43
9
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02
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12

10

Time (m:s)

8

6

4

2

0
0

0
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2
0
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4
% of concentration of enzyme

0
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6

Emma smith

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How could the controls change the investigation?
Temperature can speed up the rate of reaction by giving the enzymes and substrate more
kinetic energy so they move quicker, also if the environment is too hot it could denature the
enzyme
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Concentration of substrate affects the investigation by speeding up the rate of reaction if
there is more concentration by there being more collisions with the active site of the
enzyme with the substrate, if there is less concentration than it would be harder for the
active site to have contact with the substrate therefore slowing it down
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ConclusionOverall my hypothesis was correct, the more concentrated the solution is of the enzyme the
quicker the rate of reaction
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This is due to the higher the concentration of the enzyme means there are more
active sites in the solution to break down the casein protein
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The results from this investigation also back up my hypothesis
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The
graph shows that as the time increases the concentration decreases, meaning the lower the
concentration the more time it takes to see the x
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1% it took 10
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2% the time was at 9
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1% it took 21 seconds
quicker to break down
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Furthermore
this investigation backed up my hypothesis and proved it to be correct
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Emma smith
Lastly there were no anomalies, and all the result fit in the pattern
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I had no instrument or
solution to measure and check that each solution had the same Ph, therefore the ph could of
affected my investigation, making my results less reliable and reproducible
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Furthermore the major issue within this investigation is individual differences with your
eyesight
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This therefore will change the results, as its not valid and a good
measure as its opinionated, however to reduce this affect the results try to use the same
person to view the x

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