Notesale: Turn your study into money

Document Preview

Extracts from the notes are below, to see the PDF you'll receive please use the links above

---

Lecture 1:
In reality water does not exist in the form of H20 it exists in the form of the hydronium ion
...
The hydronium ion is formed by an
acid in water
...
Amino acids form
proteins
...
There
are 20 amino acids in animal cells
...
All the other 11 can be
biosynthesized by the cell
...
This means that one of those intermediates will be used to biosynthesis
an amino acid
...
Before they can
become useful the NH2 has to be gotten rid by deamination
...

If you look at PH you see different ranges of all the 20 amino acids (4
...
What is the reason for this
range? For some amino acids, it’s the cooh group and for some of them it goes to the high PH side due
to the amino group
...
Now, let’s look at how we can classify them
...

Another thing is that as these amino acids are chained together it gives the protein a specific function
...
) You have membrane channel proteins that are integrated into the membrane
...
So you have to have a
channel that is nonpolar, water cannot go through but nonpolar molecules or electrically neutral
molecules can go through
...
) Nonpolar amino acids if you put it in a sequence is also important in
ligand-receptor interaction
...
Ligand can be hydrophobic or
hydrophilic
...
It is not going to
bind to a protein receptor if the binding site is hydrophilic
...

So this is the functional importance of the amino acids being hydrophobic or hydrophilic
...
So they are very polar and can
dissolve in water and if they are part of a sequence they will add a hydrophilic quality to that protein
...
And also you
have Polar Basic
...
So your understanding the amino acid sequence can help you to have a prediction of the 3dimensional structure
...
When the amino acids are linked together there is the C terminal end and the N-terminal end
...


However there are C-terminals that in a lot of protein transport that is being transported first
...

How do you determine the PH of a protein as the summation of the whole group of the PH’s coming
from the different amino acids? So you determine this Isoelectric point by putting it on a gradient gel
...

What is a motif? If you look at different proteins you will repeatedly find a sequence of amino acids that
is a motif
...




---